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| Status |
Public on Nov 03, 2008 |
| Title |
Gene expression profiling of liver from dairy cows subjected to intra-mammary LPS treatment: time course |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
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| Summary |
Liver plays a profound role in the acute phase response (APR) observed in the early phase of acute bovine mastitis caused by Escherichia coli (E. coli). To gain an insight into the genes and pathways involved in hepatic APR of dairy cows we performed a global gene expression analysis of liver tissue sampled at different time points before and after intra-mammary (IM) exposure to E. coli lipopolysaccharide (LPS) treatment.
Keywords: Time course
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| Overall design |
Eight healthy, high yielding Holstein-Friesian dairy cows in their first lactation (9 to 12 weeks after calving) were chosen for this study. At time 0 the right front quarter was infused with 200 μg E. coli LPS dissolved in 10 ml 0.9% NaCl solution, the left front quarter serving as control was infused with 10 ml 0.9% NaCl solution. Liver biopsies were taken at –22, 3, 6, 9, 12 and 48 hours relative to LPS infusion in 4 cows, and also at –22, 9 and 48 hours in the remaining 4 cows. RNA from liver biopsies was isolated and biotin labeled cRNA was loaded onto the Affymetric GeneChip Bovine Genome Array. A control study using cows infused with 0.9% NaCl showed that there was no effect of taking the biopsy, neither in the clinical measurement nor in the expression of a selected subset of genes. Therefore, only samples taken from the LPS treated cows were measured for the gene expression using microarrays.
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| Contributor(s) |
Jiang L, Sørensen P, Røntved C, Nielsen L, Ingvartsen KL |
| Citation(s) |
18816405 |
| Submission date |
Mar 03, 2008 |
| Last update date |
Feb 05, 2016 |
| Contact name |
Peter Sørensen |
| E-mail(s) |
pso@mbg.au.dk
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| Organization name |
Aarhus University
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| Department |
Molecular Biology and Genetics
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| Street address |
Blichers Alle 1
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| City |
Tjele |
| ZIP/Postal code |
8830 |
| Country |
Denmark |
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| Platforms (1) |
| GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
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| Samples (36)
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| GSM270491 |
Bovine liver at -22h, bio rep 4 |
| GSM270492 |
Bovine liver at -22h, bio rep 5 |
| GSM270493 |
Bovine liver at -22h, bio rep 6 |
| GSM270494 |
Bovine liver at -22h, bio rep 7 |
| GSM270495 |
Bovine liver at -22h, bio rep 8 |
| GSM270496 |
Bovine liver at 3h, bio rep 1 |
| GSM270497 |
Bovine liver at 3h, bio rep 2 |
| GSM270498 |
Bovine liver at 3h, bio rep 3 |
| GSM270499 |
Bovine liver at 3h, bio rep 4 |
| GSM270500 |
Bovine liver at 6h, bio rep 1 |
| GSM270501 |
Bovine liver at 6h, bio rep 2 |
| GSM270502 |
Bovine liver at 6h, bio rep 3 |
| GSM270503 |
Bovine liver at 6h, bio rep 4 |
| GSM270504 |
Bovine liver at 9h, bio rep 1 |
| GSM270505 |
Bovine liver at 9h, bio rep 2 |
| GSM270506 |
Bovine liver at 9h, bio rep 3 |
| GSM270507 |
Bovine liver at 9h, bio rep 4 |
| GSM270508 |
Bovine liver at 9h, bio rep 5 |
| GSM270509 |
Bovine liver at 9h, bio rep 6 |
| GSM270510 |
Bovine liver at 9h, bio rep 7 |
| GSM270511 |
Bovine liver at 9h, bio rep 8 |
| GSM270512 |
Bovine liver at 12h, bio rep 1 |
| GSM270513 |
Bovine liver at 12h, bio rep 2 |
| GSM270514 |
Bovine liver at 12h, bio rep 3 |
| GSM270515 |
Bovine liver at 12h, bio rep 4 |
| GSM270516 |
Bovine liver at 48h, bio rep 1 |
| GSM270517 |
Bovine liver at 48h, bio rep 2 |
| GSM270518 |
Bovine liver at 48h, bio rep 3 |
| GSM270519 |
Bovine liver at 48h, bio rep 4 |
| GSM270520 |
Bovine liver at 48h, bio rep 5 |
| GSM270521 |
Bovine liver at 48h, bio rep 6 |
| GSM270522 |
Bovine liver at 48h, bio rep 7 |
| GSM270523 |
Bovine liver at 48h, bio rep 8 |
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| Relations |
| BioProject |
PRJNA107569 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE10695_RAW.tar |
117.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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