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| Status |
Public on Jan 30, 2018 |
| Title |
RNA-sequencing of bulk CD19+ Thymic B cells from young (5 week) and aged (10-24 month) mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Thymic B cells have been recently shown the ability to be licensed to express Aire, a critical transcription factor involved in the expression of self-antigens in the thymus which are critical for clonal deletion of autoreactive T cells and maintenance of self-tolerance. Mutations in AIRE cause systemic autoimmunity in humans and autoimmunity with varying degrees of severity in different mouse strains. Thymic B cells have been studied in young animals, but studies of this population with age are lacking. Given the thymus undergoes age-associated atrophy in its stromal compartment, we hypothesized that aged thymic B cells may under go changes with age which may impact their ability to mediate clonal deletion of autoreactive T cells and may contribute to age-associated increases in autoimmune prevalence. By comparing the transcriptome of young and aged thymic B cells we find that Aire and transcriptional activators of Aire are signficantly decreased with age.
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| Overall design |
Thymic B cells were enriched by incubating total thymocytes with CD19-APC (Clone: MB19-1) followed by incubation with anti-APC magnetic beads (Miltenyi Biotec). Enriched cells were then sorted using a BD FACS ARIA to a minimum purity of 98.5%, RNA extracted and analyzed
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| Contributor(s) |
Cepeda S, Cantu C, Orozco S, Xiao Y, Brown Z, Semwal MK, Venables T, Anderson MS, Griffith AV |
| Citation(s) |
29386114 |
| Submission date |
Nov 17, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
|
| Phone |
2105629163
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| Organization name |
UT Health Science Center at San Antonio
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| Department |
Population Health Sciences
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| Street address |
8403 Floyd Curl Drive, MSC 7784
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| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE107112 |
RNA-sequencing of bulk CD19+ Thymic B cells from mice and humans |
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| Relations |
| BioProject |
PRJNA419007 |
| SRA |
SRP125234 |
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