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| Status |
Public on Oct 30, 2018 |
| Title |
BHLHE40 modulates gene transcription in breast cancer cells exposed to hypoxia and low glucose |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Knockdown of BHLHE40 expression significantly reduced primary tumor growth and spontaneous lung metastasis in an orthotopic xenograft model of human breast cancer. Gene expression analysis implicated a role of BHLHE40 in hypoxia-induced exosomic secretion of Heparin Binding Epidermal Growth Factor HBEGF, which promotes cell survival and invasion. BHLHE40 induces HBEGF transcription by blocking DNA binding of HDAC1 and HDAC2.
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| Overall design |
A lung metastatic subline of MDA-MB-231, designated as LM, was stably transfected with MISSION shRNA (TRCN0000232187) to knockdown BHLHE40 or control vector. The cells were cultured in medium containing low glucose (1 mg/ml) under hypoxia (1% O2) for 6 or 48 hr. Total RNA was purified by using the RNeasy kits (Qiagen). A total of 12 samples were subjected to microarray analysis, with two biological repeats for each experiment condition.
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| Contributor(s) |
Sethuraman A, Brown M, Seagroves TN, Wu Z, Pfeffer LM, Fan M |
| Citation(s) |
30285805 |
| Submission date |
Nov 23, 2017 |
| Last update date |
Oct 30, 2018 |
| Contact name |
Meiyun Fan |
| E-mail(s) |
mfan2@uthsc.edu
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| Organization name |
University of Tennessee
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| Street address |
19 S. Manassas Street
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| City |
Memphis |
| ZIP/Postal code |
38163 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (12)
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| GSM2864583 |
LM.sh2187_hypoxia/low glucose, 6hr [8508853057_F] |
| GSM2864584 |
LM_hypoxia/low glucose, 48hr [8508853057_I] |
| GSM2864585 |
LM.sh2187_hypoxia/low glucose, 48hr [8508853057_J] |
| GSM2864586 |
LM_untreated [8508853080_A] |
| GSM2864587 |
LM_hypoxia/low glucose, 6hr [8508853080_B] |
| GSM2864588 |
LM_hypoxia/low glucose, 48hr [8508853080_C] |
| GSM2864589 |
LM.sh2187_untreated [8508853080_D] |
| GSM2864590 |
LM.sh2187_hypoxia/low glucose, 6hr [8508853080_E] |
| GSM2864591 |
LM.sh2187_hypoxia/low glucose, 48hr [8508853080_F] |
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| Relations |
| BioProject |
PRJNA419652 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE107300_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE107300_non_normalized.txt.gz |
2.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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