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| Status |
Public on Jan 16, 2018 |
| Title |
Microarray analysis of gene expression profiles with or without manipulations of neuronal activity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The process of synapse turnover is regulated by specific signaling mechanisms. Various molecules that promote formation and differentiation of synapses have been identified. Some of these molecules were classified as “synapse organizers,” as they were shown to initiate differentiation of hemi-synapses when they were expressed in non-neuronal cells and co-cultured with neurons.On the other hand, little is known about the mechanisms underlying destabilization and elimination of unnecessary synapses. We used microarray profiling of dissociated hippocampal culture to identify genes in response to the down-regulation of neuronal activity in hippocampal neurons.
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| Overall design |
Cultured hippocampal neurons were prepared from embryonic day 16.5 ICR mouse embryos. Hippocampi were dissected, trypsinized, and dissociated. Cells were plated onto 35 mm-diameter culture dishes (BD Falcon) . Cells were cultured in Minimum Essential Medium with B18 supplement and 5.5% Fetal Bovine Serum (Equitech-Bio). RNA from primary cultured neurons at 12 DIV with or without pharmacological treatments (2 μM TTX for 2days ) was purified using SV Total RNA Isolation System (Promega) according to the manufacturer's instructions. After confirming the absence of RNA degradation, RNA samples were hybridized to Agilent 8X60K Whole Mouse Genome microarrays. Array data were analyzed by quantile normalization, determination of differential gene expression, and classification of different gene expression by ontology-based methods.
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| Contributor(s) |
Higashi T, Tanaka S, Iida T, Okabe S |
| Citation(s) |
29386134 |
| Submission date |
Nov 24, 2017 |
| Last update date |
Apr 17, 2018 |
| Contact name |
Takahito Higashi |
| E-mail(s) |
thigashi@m.u-tokyo.ac.jp
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| Organization name |
Graduate School of Medicine and Faculty of Medicine, The University of Tokyo
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| Department |
Department of Cellular Neurobiology
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| Street address |
7-3-1 Hongo Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA419753 |
