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| Status |
Public on Nov 30, 2018 |
| Title |
The role of Gch1 and Nos2 in the macrophage response to BCG infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Inducible nitric oxide synthase (iNOS) plays a crucial role in controlling growth of mycobacteria, presumed to be via nitric oxide (NO) mediated killing. However, NOS enzymes can also signal through NO-independent pathways, and production of NO by NOS requires the cofactor tetrahydrobiopterin (BH4). We compared Nos2-/- mice to mice with macrophage BH4 deficiency (Gch1fl/flTie2cre), due to a leukocyte-specific deletion of Gch1, to uncover the specific contribution of NO-independent NOS functions to anti-mycobacterial immunity.
We used microarrays to detail the global programme of gene expression in uninfected and BCG infected macrophages that were either deficient in iNOS (Nos2-/- vs C57bl6/J) or BH4/Gch1 (GCHfl/flTie2cre vs GCHfl/fl)
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| Overall design |
Bone Marrow Derived Macrophages (BMDM) were cultured form C57bl6/J (Nos2+/+), Nos2-/-, GCHfl/fl and GCHfl/flTie2cre bone marrow. Differentiated macrophages from 4 animals per genotype were infected with BCG (Pasteur) in the presence of interferon gamma or left uninfected for 24 hours as control samples.
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| Contributor(s) |
McNeill E, Stylianou E, McShane H, Channon KM |
| Citation(s) |
30573728 |
| Submission date |
Nov 30, 2017 |
| Last update date |
Jan 02, 2019 |
| Contact name |
Eileen McNeill |
| Organization name |
University of Oxford
|
| Department |
Radcliffe Department of Medicine
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| Lab |
Division of Cardiovascular Medicine
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| Street address |
Wellcome Trust Centre for Human Genetics, Roosevelt Drive
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| City |
Oxford |
| ZIP/Postal code |
OX3 7BN |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (31)
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| Relations |
| BioProject |
PRJNA420498 |
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