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| Status |
Public on Aug 03, 2018 |
| Title |
RNA-seq for U937 cells with or without 3 day differentiation with PMA and recovery |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Sequencing data related to our manuscript "Systematic identification of general and context-specific regulators of phagocytosis using magnetic genome-wide CRISPR screens"
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| Overall design |
Two groups of U937 cells were sequenced before and after PMA differentiation. One group carried Streptococcus pyogenes Cas9 and a safe-harbor control sgRNA, and the second group was a clonally expanded U937 line expressing GFP. Each group was separated into eight separate wells at d0, and half of the wells were treated with 50 nM PMA. At day 3, undifferentiated cells were split to prevent overcrowding, and differentiated cells were trypsinized and replated. Cells were allowed to recover for 2 additional days before cells were lysed for RNA harvest and sequencing.
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| |
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| Contributor(s) |
Bohlen CJ, Haney MS, Bassik MC |
| Citation(s) |
30397336 |
| Submission date |
Dec 01, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Christopher John Bohlen |
| E-mail(s) |
bohlenc@gene.com
|
| Organization name |
Genentech
|
| Department |
Neuroscience
|
| Lab |
Bohlen
|
| Street address |
Room 15-2006, Genentech Building 15
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA420647 |
| SRA |
SRP125932 |
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