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| Status |
Public on Dec 28, 2017 |
| Title |
miRNA-mediated targeting of human cytomegalovirus reveals biological host and viral targets of IE2 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We introduce target sites for the microRNA (miRNA) miR-142 into the 3’-untranslated region of the human cytomegalovirus (HCMV) IE2 to study the transcriptional effect of IE2 knock-down on both viral and host genes in cells that express miR-142. When comparing transcriptional data from miR-142 expressing macrophages infected with HCMV to macrophages infected with IE2-miR-142 targeted HCMV, we see a knock-down of IE2 and differential regulation of predicted viral targets of IE2 including IE1, vIL-10 and US29. We then generated fibroblasts expressing miR-142 to study the knock-down of IE2 in a different cellular system and find drastic differences in loss of IE2 on the host transcriptional profile in the two different cell types.
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| Overall design |
BW series: Fibroblasts (MRC-5) and macrophages (THP-1) infected with HCMV and miR-142 targeted HCMV for 24h to see immediate effect of IE2-knock down. CK series: Fibroblasts and macrophages infected with HCMV and miR-142 targeted HCMV for 9 days done in duplicate. EX series: miR-142 expressing fibroblasts infected with HCMV and miR-142 targeted HCMV for 9 days done in duplicate.
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| Contributor(s) |
Møller R, Panis M, Tortorella D, tenOever BR |
| Citation(s) |
29339472 |
| Submission date |
Dec 04, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Rasmus Moeller |
| E-mail(s) |
rasmus.moeller@icahn.mssm.edu
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| Phone |
9293751400
|
| Organization name |
Icahn School of Medicine at Mount Sinai
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| Department |
Microbiology
|
| Lab |
tenOever lab
|
| Street address |
1 Gustave L. Levy Place
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA421010 |
| SRA |
SRP126100 |
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