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Series GSE107746 Query DataSets for GSE107746
Status Public on Nov 20, 2018
Title Transcriptome Landscape of Human Folliculogenesis Reveals Oocytes and Granulosa Cells Interactions
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The dynamic transcriptional regulation and interactions of human germlines and surrounding somatic cells during folliculogenesis remains unknown. Using RNA-Seq analysis of human oocytes and corresponding granulosa cells (GCs) spanning five follicular stages, we revealed unique features in transcriptional machinery, transcription factor networks and reciprocal interactions in human oocytes and GCs that displayed developmental-stage-specific expression patterns. Notably, we identified specific gene signatures of two cell types in particular developmental stage that may reflect developmental competency and ovarian reserve. Additionally, we uncovered key pathways that may concert germline-somatic interactions and drive the transition of primordial-to-primary follicle which represents follicle activation. Thus, our work provides key insights into the crucial features of the transcriptional regulation in the stepwise folliculogenesis and offers important clues for improving follicle recruitment in-vivo and restoring fully competent oocytes in-vitro.
 
Overall design Transcriptome profiles of oocytes and granulosa cells from five follicle development stages were generated by deep sequencing using illumina Hiseq X10.
 
Contributor(s) Zhang Y, Yan Z, Qiao J
Citation(s) 30472193
Submission date Dec 06, 2017
Last update date Apr 03, 2020
Contact name Zhiqiang Yan
E-mail(s) zqyan@pku.edu.cn
Organization name Peking University
Street address No.5 Yiheyuan Road Haidian District
City Beijing
ZIP/Postal code 100871
Country China
 
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (148)
GSM2877811 Primordial_follicle_A10O
GSM2877812 Primordial_follicle_A16O
GSM2877813 Primordial_follicle_A6O
Relations
BioProject PRJNA421274
SRA SRP126216

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE107746_Folliculogenesis_FPKM.log2.txt.gz 16.9 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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