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| Status |
Public on Feb 28, 2020 |
| Title |
RNA-sequencing of NNK-exposed Gprc5a-/- lung tumors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have previously found that tobacco carcinogen exposed Gprc5a-/- develop lung tumors including adenocarcinoma. We sought to understand the molecular pathology of these lung tumors by whole-transcriptome sequence (RNA-Seq) analysis.
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| Overall design |
Gprc5a-/- mice were intraperitoneally administered 50 mg/kg body weight nicotine-derived nitrosamine ketone (NNK) three times per week for 8 weeks. Mice were followed up and different groups of mice were euthanised at four to seven months following NNK. Six lung tumors that developed from lungs of five Gprc5a-/- mice at four to seven months post-NNK along with their respective five paired normal lung tissues (n=11 samples total) were sequenced.
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| Contributor(s) |
Sivakumar S, Kadara H |
| Citation(s) |
29382653 |
| Submission date |
Dec 06, 2017 |
| Last update date |
Feb 28, 2020 |
| Contact name |
Humam Kadara |
| Organization name |
University of Texas MD Anderson Cancer Center
|
| Street address |
2130 W. Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA421345 |
| SRA |
SRP126283 |
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