 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 09, 2017 |
| Title |
Transcriptomic analysis of glioblastoma cells bearing different IRE1a mutants |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Glioblastoma multiforme is the most lethal form of glioma with an overall survival at 5 years nearly null, which mainly results from acquired resistance to therapies. Large scale sequencing studies on human cancer biopsies defined IRE1alpha as the fifth most oncogenic mutated kinase in human cancer. IRE1alpha is a major component of the Unfolded Protein Response signaling and increasing evidence suggests that it is a central player in GBM development.
|
| |
|
| Overall design |
U87-MG glioblastoma cells were transfected with different IRE1a mutants or a Dominant Negative form of IRE1a. Cells transfected with Empty Vector were used as control. Cells were treated both in basal and stress conditions. For stress condition, tunicamycin (purchased from Calbiochem (Merck KGaA, Darmstadt, Germany)) was used at 0.5 µg/mL for 16 hrs. There are 3 replicates for each condition, except from the Dominant Negative (2 replicates).
|
| |
|
| Contributor(s) |
Lhomond S, Voutetakis K, Papadodima O, Logotheti M, Chatziioannou A, Chevet E |
| Citation(s) |
29311133 |
| Submission date |
Dec 08, 2017 |
| Last update date |
Apr 20, 2018 |
| Contact name |
Aristotelis Chatziioannou |
| E-mail(s) |
achatzi@eie.gr
|
| Organization name |
National Hellenic Research Foundation
|
| Department |
Institute of Biology, Medicinal Chemistry and Biotechnology
|
| Lab |
Metabolic Engineering - Bioinformatics
|
| Street address |
48 Vas. Constantinou Ave.
|
| City |
Athens |
| ZIP/Postal code |
11635 |
| Country |
Greece |
| |
|
| Platforms (1) |
| GPL13158 |
[HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate |
|
| Samples (40)
|
| GSM2882143 |
clone 3 stressed, biological rep1 |
| GSM2882144 |
clone 3 stressed, biological rep2 |
| GSM2882145 |
clone 3 stressed, biological rep3 |
| GSM2882146 |
clone 4, biological rep1 |
| GSM2882147 |
clone 4, biological rep2 |
| GSM2882148 |
clone 4, biological rep3 |
| GSM2882149 |
clone 4 stressed, biological rep1 |
| GSM2882150 |
clone 4 stressed, biological rep2 |
| GSM2882151 |
clone 4 stressed, biological rep3 |
| GSM2882152 |
clone 6, biological rep1 |
| GSM2882153 |
clone 6, biological rep2 |
| GSM2882154 |
clone 6, biological rep3 |
| GSM2882155 |
clone 6 stressed, biological rep1 |
| GSM2882156 |
clone 6 stressed, biological rep2 |
| GSM2882157 |
clone 6 stressed, biological rep3 |
| GSM2882158 |
clone 7, biological rep1 |
| GSM2882159 |
clone 7, biological rep2 |
| GSM2882160 |
clone 7, biological rep3 |
| GSM2882161 |
clone 7 stressed, biological rep1 |
| GSM2882162 |
clone 7 stressed, biological rep2 |
| GSM2882163 |
clone 7 stressed, biological rep3 |
| GSM2882164 |
Dominant negative, biological rep2 |
| GSM2882165 |
Dominant negative, biological rep3 |
| GSM2882166 |
Dominant negative stressed, biological rep2 |
| GSM2882167 |
Dominant negative stressed, biological rep3 |
| GSM2882168 |
Empty vector, biological rep1 |
| GSM2882169 |
Empty vector, biological rep2 |
| GSM2882170 |
Empty vector, biological rep3 |
| GSM2882171 |
Empty vector stressed, biological rep1 |
| GSM2882172 |
Empty vector stressed, biological rep2 |
| GSM2882173 |
Empty vector stressed, biological rep3 |
| GSM2882174 |
Wild type, biological rep1 |
| GSM2882175 |
Wild type, biological rep2 |
| GSM2882176 |
Wild type, biological rep3 |
| GSM2882177 |
Wild type stressed, biological rep1 |
| GSM2882178 |
Wild type stressed, biological rep2 |
| GSM2882179 |
Wild type stressed, biological rep3 |
|
| Relations |
| BioProject |
PRJNA421725 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE107859_RAW.tar |
88.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...