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| Status |
Public on Dec 12, 2017 |
| Title |
Characterization of distinct states of mouse naïve and primed pluriopotency |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Pluripotency of mammalian stem cells is stabilized at different status depending upon the extracellular stimuli of their environment. Now, there is a consensus that mouse embryonic stem cells (mESCs) and iPSCs (miPSCs) represent undifferentiated status very close to ground-state of mammalian development when cultured in optimal condition. Since they have not been activated to commit into any lineages, they are stated as “naïve-state stem cells”. In contrast, human ESCs (hESCs), hiPSCs, and mEpiSCs are considered to possess partially differentiated characteristics compared with mESCs and miPSCs. Thus, they are stated as “primed-state stem cells”. Investigation of the prerequisites for establishment of the naïve-state is significantly important to fully understand mammalian development and to extrapolate the technologies developed with mouse ES/iPS cells for human.
We used expression data to understand the gene expression profile and identified distinct characteristics on pluripotent state.
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| Overall design |
Mouse embryonic stem cell and epiblast stem cell were traditionally maintained in naïve and primed-state. And also, we obtained converted naïve-state pluripotent stem cell. Characteristics analysis was perfomed to investigate and compare the gene expression profile in three samples.
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| Contributor(s) |
Takehara T, Teramura T, Suemori H |
| Citation missing |
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| Submission date |
Dec 11, 2017 |
| Last update date |
Feb 01, 2019 |
| Contact name |
Toshiyuki Takehara |
| E-mail(s) |
takehara@med.kindai.ac.jp
|
| Organization name |
Kindai Universty Hospital
|
| Department |
Faculty of Medicine
|
| Lab |
Regenerative Medicine
|
| Street address |
377-2, Ohno-Higashi
|
| City |
Osaka-Sayama |
| State/province |
Osaka |
| ZIP/Postal code |
5898511 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (6)
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| GSM2882838 |
mouse epiblast stem cell rep2 |
| GSM2882839 |
converted naïve state pluripotent stem cell rep1 |
| GSM2882840 |
converted naïve state pluripotent stem cell rep2 |
|
| Relations |
| BioProject |
PRJNA421878 |
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