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| Status |
Public on Aug 24, 2018 |
| Title |
Identification of Glucocorticoid-Induced Leucine Zipper (Gilz) gene targets in undifferentiated spermatogonia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Sustained spermatogenesis in adult males and recovery of fertility following germ cell depletion are dependent on undifferentiated spermatogonia with self-renewal potential. We have previously demonstrated a critical cell-autonomous role for Gilz in spermatogonial stem cell maintainance and spermatogenesis. To identify genes regulated by Gilz in the male germline, we have isolated undifferentiated spermatogonial cells from tamoxifen treated Gilzflox/flox (Control) and Gilzflox/flox UBC-CreER (TAM-KO) mice that will allow identification of genes mis-expressed upon loss of GILZ.
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| Overall design |
4 independent sets of Gilzflox/flox (Control) and Gilzflox/flox UBC-CreER (TAM-KO) undifferentiated spermatogonia were isolated by flow sorting from adult mouse testes 7 days after treatment with tamoxifen.
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| Contributor(s) |
Hobbs RM, La HM, Chan A, Legrand JM |
| Citation(s) |
30126904 |
| Submission date |
Dec 11, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Robin Mark Hobbs |
| E-mail(s) |
robin.hobbs@monash.edu
|
| Organization name |
Hudson Institute of Medical Research
|
| Department |
Centre for Reproductive Health
|
| Lab |
Germline Stem Cell Biology Laboratory
|
| Street address |
27-31 Wright Street
|
| City |
CLAYTON |
| State/province |
VIC |
| ZIP/Postal code |
3168 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (8)
|
| GSM2882955 |
Gilzflox/flox (Control) spermatogonia rep 1 |
| GSM2882956 |
Gilzflox/flox UBC-CreER (TAM-KO) spermatogonia rep 1 |
| GSM2882957 |
Gilzflox/flox (Control) spermatogonia rep 2 |
| GSM2882958 |
Gilzflox/flox UBC-CreER (TAM-KO) spermatogonia rep 2 |
| GSM2882959 |
Gilzflox/flox (Control) spermatogonia rep 3 |
| GSM2882960 |
Gilzflox/flox UBC-CreER (TAM-KO) spermatogonia rep 3 |
| GSM2882961 |
Gilzflox/flox (Control) spermatogonia rep 4 |
| GSM2882962 |
Gilzflox/flox UBC-CreER (TAM-KO) spermatogonia rep 4 |
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| Relations |
| BioProject |
PRJNA421886 |
| SRA |
SRP126482 |
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