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| Status |
Public on Dec 01, 2020 |
| Title |
Astrocytes respond to a reduction of extracellular Nerve Growth Factor levels by a phosphorylation of TrkA in the endoplasmic reticulum: a microarray transcriptome study |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The discovery that other cells besides neurons, like the glia cells, could respond to NGF and modulate the neuronal physiology broadened the possible NGF targets to potentially all the brain, boosting the comprehension of neuronal pathologies - like Alzheimer's disease - and supporting more therapeutical strategies. This microarray transcriptome analysis of primary astrocytes was performed to demonstrate a direct modulation of NGF activity on astrocyte cells. We investigated this modulation by neutralizing extracellular NGF by the alfaD11 monoclonal antibody and measuring the gene expression profile. Data analysis was performed using R-Bioconductor.
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| Overall design |
A total of n=8 samples were processed. The samples are divided into 3 experimental groups: anti-NGF alfaD11 antibody treated samples (n=4), naive cells control samples (n=2), anti-V5 antibody treated control samples (n=2). Samples were treated for 24h.
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| Contributor(s) |
Carucci N, Testa G, Gobbo F, Rizzi C, Galli-Resta L, Arisi I, D'Onofrio M, Brandi R, Capsoni S, Cattaneo A |
| Citation(s) |
37143894 |
| Submission date |
Dec 14, 2017 |
| Last update date |
Aug 02, 2023 |
| Contact name |
Ivan Arisi |
| E-mail(s) |
i.arisi@ebri.it
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| Phone |
+39-06-49255230
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| Organization name |
European Brain Research Institute
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| Department |
Bioinformatics Facility
|
| Street address |
viale Regina Elena 295
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| City |
Roma |
| ZIP/Postal code |
00161 |
| Country |
Italy |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA422452 |
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