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Series GSE108144 Query DataSets for GSE108144
Status Public on Oct 05, 2018
Title TRIM28 protects TRIM24 from SPOP-mediated degradation and promotes prostate cancer progression [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In this study, proteomic profiling of TRIM24 interactome in conjunction with shRNA screening of TRIM24 top-interactors nominated that TRIM28 is indispensable for TRIM24 protein stability. We showed that TRIM28 stabilizes TRIM24 against SPOP-mediated ubiquitination and degradation. TRIM28 promotes TRIM24 and AR transcription activity, androgen-dependent and -independent PCa growth. In addition, we demonstrated that TRIM28 level in high in advanced PCa, which drives TRIM24/AR transcription activity in a similar manner to SPOP mutation, which implies that TRIM28 potentially dictates the therapeutic outcome of TRIM24-targeted therapy.
 
Overall design genetic modification design
 
Contributor(s) Yu J, Fong K
Citation(s) 30479348
Submission date Dec 15, 2017
Last update date Feb 04, 2020
Contact name Jindan Yu
E-mail(s) jindan.yu@emory.edu
Organization name Emory University
Department Urology
Lab Jindan Yu's lab
Street address E330, 1760 Haygood Dr NE
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platforms (1)
GPL15456 Illumina HiScanSQ (Homo sapiens)
Samples (7)
GSM2891161 LNCaP HA-TRIM24 + Ethl
GSM2891162 LNCaP HA-TRIM24 + 10nM R1881
GSM2891163 LNCaP TRIM28 + Ethl
This SubSeries is part of SuperSeries:
GSE108146 TRIM28 protects TRIM24 from SPOP-mediated degradation and promotes prostate cancer progression
Relations
BioProject PRJNA422654
SRA SRP126894

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108144_RAW.tar 351.3 Mb (http)(custom) TAR (of BEDGRAPH, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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