|
| Status |
Public on Dec 30, 2019 |
| Title |
Microbial activities associated with human intestinal mucosal biofilms change mRNAs and microRNAs to promote carcinogenesis in mice [RNA] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Disrupted interactions between host and intestinal bacteria are implicated in the development of colorectal cancer (CRC). However, the functional impacts of these inter-kingdom interactions remain poorly defined. To examine this interplay, we performed mouse and microbiota RNA-sequencing on colon tissue from germ-free (GF) and gnotobiotic ApcMin/+;Il10-/- mice associated with microbes from biofilm-positive human CRC tumor (BT) and biofilm-negative healthy (BX) tissues. The bacteria in BT mice differentially expressed >2,900 genes related to bacterial secretion, virulence and biofilms, but only affected 62 host genes. Importantly, the bacterial communities from BT mice were transmissible and carcinogenic when administered to a new GF ApcMin/+;Il10-/- cohort, maintaining a set of 13 bacterial genera. Our findings suggest complex interactions within bacterial communities affecting bacterial composition and CRC development.
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| Overall design |
RNA-sequencing on colon tissue from germ-free (GF) and gnotobiotic ApcMin/+;Il10-/- mice associated with microbes from biofilm-positive human CRC tumor (BT) and biofilm-negative healthy (BX) tissues.
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| |
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| Contributor(s) |
Sears C, Jobin C |
| Citation(s) |
31937674 |
| Submission date |
Dec 15, 2017 |
| Last update date |
Feb 10, 2020 |
| Contact name |
Christian Jobin |
| Organization name |
University of Florida
|
| Department |
Medicine
|
| Street address |
2033 Mowry Rd, CGRC-461, PO Box: 103633
|
| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE108165 |
Microbial activities associated with human intestinal mucosal biofilms change mRNAs and microRNAs to promote carcinogenesis in mice |
|
| Relations |
| BioProject |
PRJNA422619 |
| SRA |
SRP126873 |
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