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Series GSE10823 Query DataSets for GSE10823
Status Public on Dec 25, 2008
Title Species difference in gene expression effect of trichloroethylene (TCE) between mouse and rat (rat)
Organisms Mus musculus; Rattus norvegicus
Experiment type Expression profiling by array
Summary TCE is a non-genotoxic hepatocarcinogen in mouse, but not in rat or human. Extrapolation of data from laboratory animals to humans is difficult due to species-specific differences. To identify molecular pathways and biological changes responsible for species-specific differences in hepatocarcinogenesis, we analyzed gene expression profiles of livers from B6C3F1 mice and SD rats administered TCE by oral gavage once or repeatedly every 24 hrs for 14 days. Gene expression analysis revealed distinct clusters of transcriptional profiles in single- and repeated-dose mice and rats. Pathway analysis showed differences in biological pathways between single- and repeated-dose mice and rats. Activation of the MAPK signaling cascade and ubiquitin-proteasome inhibitory function, as well as inhibition of TGF-beta signaling, were specific to mice and suggest a role in hepatocyte proliferation. Although pathological analysis showed no evidence of apoptosis, gene expression analysis revealed changes in apoptosis-related genes. In addition to the previously reported suppression of apoptosis, results in repeated-dose mice showed that toxicity induced by TCE in turn induces apoptosis.
Keywords: Response to chemical
 
Overall design Eight-week-old B6C3F1 mice and SD rats were administered 1500mg/kgBW TCE (n=4) or with corn oil (n=4) once or every 24h for 2 weeks by oral gavage. The liver tissue were collected 18h after the last dosage. mRNA were extracted from the liver and were hybridized to Affymetrix GeneChip
 
Contributor(s) Sano Y, Nakashima H, Yoshioka N, Omae K
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Submission date Mar 14, 2008
Last update date Feb 18, 2018
Contact name Sano Yuri
E-mail(s) sanoyuri@sc.itc.keio.ac.jp
Phone +3 5363 3758
Fax +3 3359 3686
URL http://www.keiopublichealth.jp/
Organization name Keio Univ.
Department Department of Preventive Medicine and Public Health
Street address 35 Shinanomachi
City Shinjyuku-ku
State/province Tokyo
ZIP/Postal code 160-8582
Country Japan
 
Platforms (2)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
GPL85 [RG_U34A] Affymetrix Rat Genome U34 Array
Samples (32)
GSM273237 Mouse_liver_single-dose_corn oil_rep1
GSM273238 Mouse_liver_single-dose_corn oil_rep2
GSM273239 Mouse_liver_single-dose_corn oil_rep3
Relations
BioProject PRJNA107427

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10823_RAW.tar 74.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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