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Series GSE108717 Query DataSets for GSE108717
Status Public on Apr 28, 2018
Title Setd2-dependent H3K36me3 is required for Acrbp1 and protamine transcriptions and spermiogenesis in mice
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Spermatogenesis is precisely cotrolled by complex gene expression programs and involves epigenetic reprogramming including histone modification and DNA methylation. Setd2 catalyzes the trimethylation of histone H3 Lys36 (H3K36me3) and plays key roles in embryonic stem cell differentiation and somatic cell development; however, its role in male germ cell development remains elusive. Here we demonstrate an essential role of Setd2 for spermiogenesis. We show that targeted knockout of Setd2 in germ cells causes aberrant spermiogenesis with acrosomal malformation before step 8 round spermatid stage, resulting in complete male infertile. Furthermore, we show a complete loss of H3K36me3 and a significant altered gene expression profile, including Acrbp1 and protamines, caused by Setd2 deficiency. Our findings reveal a previously underappreciated role of Setd2-dependent H3K36me3 for spermiogenesis and improved the understanding of epigenetic disorders underlying male infertility.
 
Overall design We examined H3K36me3 and transcriptomes in in PD and RS cells upon Setd2 loss.
 
Contributor(s) Rong B, Li L, Tong M, Lan F, Lyu R
Citation(s) 29716999
Submission date Jan 03, 2018
Last update date Mar 21, 2019
Contact name Fei Lan
E-mail(s) fei_lan@fudan.edu.cn
Organization name Institute of Biochemical Science
Lab Epigenetics lab
Street address No131 Dongan Road, Xuhui District.
City Shanghai
ZIP/Postal code 200030
Country China
 
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (14)
GSM2912475 PD_WT_H3K36me3
GSM2912476 PD_WT_Input
GSM2912477 PD_Setd2KO_H3K36me3
Relations
BioProject PRJNA428362
SRA SRP127980

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108717_PD_Setd2KO_RNA-seq_FPKM.txt.gz 502.5 Kb (ftp)(http) TXT
GSE108717_PD_WT_RNA-seq_FPKM.txt.gz 491.1 Kb (ftp)(http) TXT
GSE108717_RAW.tar 859.8 Mb (http)(custom) TAR (of BED, BEDGRAPH)
GSE108717_RS_Setd2KO_RNA-seq_FPKM.txt.gz 513.1 Kb (ftp)(http) TXT
GSE108717_RS_WT_RNA-seq_FPKM.txt.gz 505.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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