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| Status |
Public on Mar 26, 2018 |
| Title |
ATAC-Seq analysis in vasopressin-responsive mouse renal collecting duct mpkCCD cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Vasopressin, a peptide hormone, controls renal water excretion, largely through regulation of water channel aquaporin-2 (AQP2) in the renal collecting duct. There are two regulatory mechanisms of AQP2: 1) short-term regulation by membrane trafficking of AQP2; and 2) long-term regulation involving vasopressin-induced changes of protein abundance of AQP2 through regulation of gene transcription and protein half-life. Vasopressin binds a G protein-coupled receptor (V2R) activating several downstream signaling pathways. At downstream of V2R activation, many of transcription factors involve gene transcription process associated with status of chromatin structure. ATAC-Seq (Assay for Transpoase-Accessible Chromatin using Sequencing) is a recent technique to study chromatin accessibility (Buenrostro et al. Nat Methods 2013). We carried out ATAC-Seq following standard an ATAC-Seq protocol in mpkCCD cells treated with vehicle or dDAVP for 30 minutes.
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| Overall design |
To identify Tn5-accessible chromatin regions where transcription factors may bind, we carried out ATAC-seq . Observations were made both after 30 minutes treatment with the vasopressin analog dDAVP or vehicle (n=2 for each group).
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| Contributor(s) |
Jung H, Knepper MA |
| Citation(s) |
29572403 |
| Submission date |
Jan 04, 2018 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Hyun Jun Jung |
| E-mail(s) |
hjung24@jhmi.edu
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Medicine (Nephrology)
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| Street address |
720 Rutland Ave, Ross Research Building 1165B
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA428530 |
| SRA |
SRP130938 |
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