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| Status |
Public on Dec 17, 2008 |
| Title |
Transcription profile of CD103- vs CD103+ dendritic cells derived from CX3CR1+c-kit+- bone marrow cells (M1993 M2067) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We hypothesize that under homeostatic as well as inflammatory conditions circulating monocytes and/or their bone marrow-derived progenitors might contribute to the replenishment of CD103+ and CD103- DC in lymphoid and non-lymphoid compartments. To that end, bone marrow cells from CX3CR1+/gfp C57BL/6 mice were sorted as follows: lineage negative (CD3, CD19, NK1.1, Ter119, Ly6G and CD11c) CX3CR1+c-kit+. Sorted cells were further cultured in vitro under the continuous presence of GM-CSF. lin-CX3CR1+c-kit+ bone marrow cells gave rise to CD103+ and CD103- DC in vitro. To test whether CD103 might be a suitable marker that allows the differentiation of functionally distinct DC subsets generated in vitro, CD11c+CD103+ and CD11c+CD103- DC were sorted from day 5 cultures of lin-CX3CR1+c-kit+ cells and analyzed by whole mouse genome microarrays from Agilent technologies. Compared to CD103+ DC, CD103- DC displayed a strong up-regulation of transcripts for genes involved in innate immunity, whereas those involved in costimulation were down-modulated. Our data suggest distinct functional activity of these two DC subsets.
Keywords: Transcriptional profiling-Cell type comparison
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| Overall design |
We compared the transcriptional profile of CD11c+CD103+ and CD11c+CD103- dendritic cells by use of Agilent Whole Mouse Genome Microarrays. Two sets of samples were generated independently as real biological replicates. The microarray analysis was performed as a dual-color experiment, including a dye-swap.
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| Contributor(s) |
del Rio ML, Rodriguez-Barbosa J, Bölter J, Ballmaier M, Dittrich-Breiholz O, Kracht M, Jung S, Förster R |
| Citation(s) |
18941208 |
| Submission date |
Mar 18, 2008 |
| Last update date |
May 10, 2018 |
| Contact name |
Oliver Dittrich-Breiholz |
| E-mail(s) |
dittrich.oliver@mh-hannover.de
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| Organization name |
Medical School Hannover
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| Department |
Research Core Unit Genomics
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| Street address |
Carl-Neuberg-Str. 1
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| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (2) |
| GSM275765 |
CD11c+CD103+ versus CD11c+CD103- dendritic cells_replicate1_M1993 |
| GSM275767 |
CD11c+CD103+ versus CD11c+CD103- dendritic cells_replicate2_M2067 |
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| Relations |
| BioProject |
PRJNA107275 |
