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| Status |
Public on Jan 31, 2018 |
| Title |
PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of mast cell signalling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
PEST-domain-enriched tyrosine phosphatase (PEP) is a cytoplasmic protein tyrosine phosphatase that regulates immune cell functions, including mast cell functions. Using bone marrow derived mast cells (BMMCs) from PEP+/+ and PEP-/- mice, RNA-seq data showed that dinitrophenol (DNP) - activated PEP-/- BMMCs have misregulated gene expression, with some cytokine/chemokine genes (eg.TNFα, IL13, CSF2) showing reduced gene expression in the dinitrophenol (DNP) - activated PEP-/- BMMCs compared to (DNP)-activated PEP+/+ BMMCs. Also, the ability of the glucocorticoid dexamethasone (Dex) to negatively regulate DNP - induced COX-2 gene expression in PEP-/- BMMCs was inhibited compared to the PEP+/+ BMMCs.
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| Overall design |
Biological replicates are sequenced and analyzed. The samples are either wild-type or mutant for PEP and cells were sensitized with Ig-E, activated with Dinitrophenol and glucocorticoid treatment done with Dexamethasone.
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| Contributor(s) |
Ainooson GK, Gourain V, Cato AC |
| Citation(s) |
30238026 |
| Submission date |
Jan 09, 2018 |
| Last update date |
Oct 16, 2018 |
| Contact name |
Victor GOURAIN |
| Organization name |
Centre de recherche en transplantation et immunologie
|
| Department |
UMR1064
|
| Street address |
30 Bd Jean Monnet
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| City |
Nantes |
| ZIP/Postal code |
44093 |
| Country |
France |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA429265 |
| SRA |
SRP128580 |
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