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| Status |
Public on Jan 11, 2018 |
| Title |
The satellite cell niche regulates the balance between myoblast differentiation and self-renewal via p53 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Satellite cells (SCs) are adult muscle stem cells residing in a specialised niche that regulates SC homeostasis. How niche-generated signals integrate to regulate gene expression in SC-derived myoblasts, is poorly understood. We undertook an unbiased approach to study the effect of the SC niche on SC-derived myoblast transcriptional regulation and identified the tumour suppressor p53 as a key player in the regulation of myoblast quiescence. After activation and proliferation, a subpopulation of myoblasts cultured in the presence of the niche upregulates p53 and fails to differentiate. When SC self-renewal is modelled ex vivo in a reserve cell assay, myoblasts treated with Nutlin-3, which increases p53 levels in the cell, fail to differentiate and instead become quiescent. Since both these effects of Nutlin-3 are rescued by siRNA-mediated p53 knockdown we conclude that a tight control of p53 levels in myoblasts regulate the balance between differentiation and return to quiescence.
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| Overall design |
Myofibres were extracted from Male mice and 48h and 72h after isolation collected washed and characterized on Agilent microarrays.
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| Contributor(s) |
Flamini V, Ghadiali RS, Antczak P, Rothwell A, Turnbull JE, Pisconti A |
| Citation(s) |
29429962 |
| Submission date |
Jan 10, 2018 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Philipp Antczak |
| E-mail(s) |
p.antczak@liv.ac.uk
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| Organization name |
University of Liverpool
|
| Street address |
Crown Street
|
| City |
Liverpool |
| State/province |
Merseyside |
| ZIP/Postal code |
L69 7ZB |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA429482 |
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