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| Status |
Public on Oct 08, 2018 |
| Title |
Gene expression in adult sciatic nerves upon miRNA ablation and crush injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report RNA sequencing data from induced Schwann cell specific knockouts for DICER and DGCR8 as well as crush injured wild type animals. Induced deletion of DICER and DGCR8 results in expression of numerous injury response genes suggesting a requirement of those proteins in maintaining the myelinated Schwann cell fate.
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| Overall design |
Sciatic nerve RNA expression profiles of adult 3 months post tamoxifen induced P0-CreERT2-Dicer and P0-CreERT2-Dgcr8 mice (in triplicate) and C57BL6 3 days post crush injury (in quadruplicate) in comparison to contralateral (in quadruplicate) and uncrushed controls (in triplicate) were generated by deep sequencing, using Illumina HiSeq.
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| Contributor(s) |
Goekbuget D, Pereira JA, Opitz L, Suter U |
| Citation(s) |
30295958 |
| Submission date |
Jan 11, 2018 |
| Last update date |
Jul 11, 2022 |
| Contact name |
Robert Blelloch |
| Organization name |
UCSF
|
| Department |
Urology
|
| Street address |
35 Medical Center Way
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (23)
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| This SubSeries is part of SuperSeries: |
| GSE109075 |
Transcriptome analyses of miRNA pathway mutants and injury response in developing and adult sciatic nerves |
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| Relations |
| BioProject |
PRJNA429699 |
| SRA |
SRP129055 |
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