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| Status |
Public on Oct 08, 2018 |
| Title |
Transcriptome analyses of miRNA pathway mutants and injury response in developing and adult sciatic nerves |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We addressed the requirement of DGCR8, DROSHA and DICER functions in developing and adult Schwann cells (SCs) using mouse mutants. We found that the microprocessor components DGCR8 and DROSHA are crucial for axonal radial sorting and to establish correct SC numbers upon myelination. Transcriptome analysis revealed that the microprocessor is essential to prevent aberrant accumulation and de novo expression of injury-response genes. Those genes are predicted targets of stage-specifically enriched miRNAs. In agreement, DGCR8 and DICER are required for proper maintenance of the myelinated SC state. We conclude that the miRNA pathway is crucial for preventing inappropriate activity of injury response genes in developing and adult SCs.
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| Overall design |
At post natal day 1, sciatic nerves of Schwann cell specific conditional mutants for DICER, DGCR8, DROSHA and respective controls were analyzed by RNA sequencing in triplicate.
Sciatic nerves of induced Schwann cell specific mutants for DICER and DGCR8 were analyzed 3 months after administration of tamoxifen and compared to P0-CreERT2-negative controls in triplicate. In addition, wild-type adult sciatic nerves were analyzed 3 days post crush injury (in quadruplicate) in comparison to contralateral (in quadruplicate) and uncrushed sciatic nerve controls (in triplicate).
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| Citation(s) |
30295958 |
| Submission date |
Jan 11, 2018 |
| Last update date |
Jul 11, 2022 |
| Contact name |
Robert Blelloch |
| Organization name |
UCSF
|
| Department |
Urology
|
| Street address |
35 Medical Center Way
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (38)
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| This SuperSeries is composed of the following SubSeries: |
| GSE64880 |
RNA expression in mutants of the miRNA pathway during myelination |
| GSE109074 |
Gene expression in adult sciatic nerves upon miRNA ablation and crush injury |
|
| Relations |
| BioProject |
PRJNA429613 |
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