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| Status |
Public on May 18, 2018 |
| Title |
1-day styrene inhalation dose response in three strains of C57Bl/6 mice: wild-type (WT), CYP2F2 knockout (KO), and CYP2F1 humanized (TG) mice, and CD-1 mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
These data examine transcriptomic changes in Lung tissue after a single day's exposure to Styrene inhalation in male C57Bl/6 and CD-1 mice. Styrene causes increased lung tumors in mice, but not in rats. Mouse lung tumors were found mostly at the conclusion of a life-time (104 weeks for males) exposure study and most were benign. Styrene is largely negative in genotoxicity assays. Styrene metabolism by CYP2F2 produced a different metabolite pattern in mouse lung than in liver or in rats or humans. The purpose of this study was to use genomic analyses to further investigate potential modes of action (MoA) of styrene in mice after a short term exposure to styrene. Mice strains exposed were C57Bl/6 wild-type (WT), CYP2F2 knockout (-/-; KO) and CYP2F21 humanized transgenic (2F2-KO + 2F1,2A13,2B6-transgenic, TG), and CD-1 male mice using 10, 40 & 120 ppm styrene at 6 hr/day for a single day. Lungs were analyzed by Affymetrix whole genome microarrays for each strain relative to strain specific vehicle controls for each strain. Both RMA normalization and statistical analsis (ANOVA) was performed on each strain separately. Affymetrix control probes (AFFX-prefix, 104 probes) have been excluded from the data matrix.
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| Overall design |
Lung gene expression was evaluated in male CD-1 and C57Bl/6 WT, KO & TG mice following a single 6-hr inhalation exposure of styrene at 0, 10, 40, and 120 ppm
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| Contributor(s) |
Black MB, Andersen ME |
| Citation(s) |
29777725 |
| Submission date |
Jan 11, 2018 |
| Last update date |
Aug 06, 2018 |
| Contact name |
Michael B. Black |
| E-mail(s) |
mblack@scitovation.com
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| Phone |
919-354-5223
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| Organization name |
Scitovation, LLC.
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| Street address |
100 Capitola Dr., Suite 106
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| City |
Research Triangle Park |
| State/province |
North Carolina |
| ZIP/Postal code |
27713 |
| Country |
USA |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (80)
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| GSM2931244 |
CD-1 Mouse Lung, Dose 0, 1day, biological replicate 1 |
| GSM2931245 |
CD-1 Mouse Lung, Dose 0, 1day, biological replicate 2 |
| GSM2931246 |
CD-1 Mouse Lung, Dose 0, 1day, biological replicate 3 |
| GSM2931247 |
CD-1 Mouse Lung, Dose 0, 1day, biological replicate 4 |
| GSM2931248 |
CD-1 Mouse Lung, Dose 0, 1day, biological replicate 5 |
| GSM2931249 |
CD-1 Mouse Lung, Dose 10ppm, 1day, biological replicate 1 |
| GSM2931250 |
CD-1 Mouse Lung, Dose 10ppm, 1day, biological replicate 2 |
| GSM2931251 |
CD-1 Mouse Lung, Dose 10ppm, 1day, biological replicate 3 |
| GSM2931252 |
CD-1 Mouse Lung, Dose 10ppm, 1day, biological replicate 4 |
| GSM2931253 |
CD-1 Mouse Lung, Dose 10ppm, 1day, biological replicate 5 |
| GSM2931254 |
CD-1 Mouse Lung, Dose 40ppm, 1day, biological replicate 1 |
| GSM2931255 |
CD-1 Mouse Lung, Dose 40ppm, 1day, biological replicate 2 |
| GSM2931256 |
CD-1 Mouse Lung, Dose 40ppm, 1day, biological replicate 3 |
| GSM2931257 |
CD-1 Mouse Lung, Dose 40ppm, 1day, biological replicate 4 |
| GSM2931258 |
CD-1 Mouse Lung, Dose 40ppm, 1day, biological replicate 5 |
| GSM2931259 |
CD-1 Mouse Lung, Dose 120ppm, 1day, biological replicate 1 |
| GSM2931260 |
CD-1 Mouse Lung, Dose 120ppm, 1day, biological replicate 2 |
| GSM2931261 |
CD-1 Mouse Lung, Dose 120ppm, 1day, biological replicate 3 |
| GSM2931262 |
CD-1 Mouse Lung, Dose 120ppm, 1day, biological replicate 4 |
| GSM2931263 |
CD-1 Mouse Lung, Dose 120ppm, 1day, biological replicate 5 |
| GSM2931264 |
C57Bl/6 KO Mouse Lung, Dose 0, 1day, biological replicate 1 |
| GSM2931265 |
C57Bl/6 KO Mouse Lung, Dose 0, 1day, biological replicate 2 |
| GSM2931266 |
C57Bl/6 KO Mouse Lung, Dose 0, 1day, biological replicate 3 |
| GSM2931267 |
C57Bl/6 KO Mouse Lung, Dose 0, 1day, biological replicate 4 |
| GSM2931268 |
C57Bl/6 KO Mouse Lung, Dose 0, 1day, biological replicate 5 |
| GSM2931269 |
C57Bl/6 KO Mouse Lung, Dose 10ppm, 1day, biological replicate 1 |
| GSM2931270 |
C57Bl/6 KO Mouse Lung, Dose 10ppm, 1day, biological replicate 2 |
| GSM2931271 |
C57Bl/6 KO Mouse Lung, Dose 10ppm, 1day, biological replicate 3 |
| GSM2931272 |
C57Bl/6 KO Mouse Lung, Dose 10ppm, 1day, biological replicate 4 |
| GSM2931273 |
C57Bl/6 KO Mouse Lung, Dose 10ppm, 1day, biological replicate 5 |
| GSM2931274 |
C57Bl/6 KO Mouse Lung, Dose 40ppm, 1day, biological replicate 1 |
| GSM2931275 |
C57Bl/6 KO Mouse Lung, Dose 40ppm, 1day, biological replicate 2 |
| GSM2931276 |
C57Bl/6 KO Mouse Lung, Dose 40ppm, 1day, biological replicate 3 |
| GSM2931277 |
C57Bl/6 KO Mouse Lung, Dose 40ppm, 1day, biological replicate 4 |
| GSM2931278 |
C57Bl/6 KO Mouse Lung, Dose 40ppm, 1day, biological replicate 5 |
| GSM2931279 |
C57Bl/6 KO Mouse Lung, Dose 120ppm, 1day, biological replicate 1 |
| GSM2931280 |
C57Bl/6 KO Mouse Lung, Dose 120ppm, 1day, biological replicate 2 |
| GSM2931281 |
C57Bl/6 KO Mouse Lung, Dose 120ppm, 1day, biological replicate 3 |
| GSM2931282 |
C57Bl/6 KO Mouse Lung, Dose 120ppm, 1day, biological replicate 4 |
| GSM2931283 |
C57Bl/6 KO Mouse Lung, Dose 120ppm, 1day, biological replicate 5 |
| GSM2931284 |
C57Bl/6 TG Mouse Lung, Dose 0, 1day, biological replicate 1 |
| GSM2931285 |
C57Bl/6 TG Mouse Lung, Dose 0, 1day, biological replicate 2 |
| GSM2931286 |
C57Bl/6 TG Mouse Lung, Dose 0, 1day, biological replicate 3 |
| GSM2931287 |
C57Bl/6 TG Mouse Lung, Dose 0, 1day, biological replicate 4 |
| GSM2931288 |
C57Bl/6 TG Mouse Lung, Dose 0, 1day, biological replicate 5 |
| GSM2931289 |
C57Bl/6 TG Mouse Lung, Dose 10ppm, 1day, biological replicate 1 |
| GSM2931290 |
C57Bl/6 TG Mouse Lung, Dose 10ppm, 1day, biological replicate 2 |
| GSM2931291 |
C57Bl/6 TG Mouse Lung, Dose 10ppm, 1day, biological replicate 3 |
| GSM2931292 |
C57Bl/6 TG Mouse Lung, Dose 10ppm, 1day, biological replicate 4 |
| GSM2931293 |
C57Bl/6 TG Mouse Lung, Dose 10ppm, 1day, biological replicate 5 |
| GSM2931294 |
C57Bl/6 TG Mouse Lung, Dose 40ppm, 1day, biological replicate 1 |
| GSM2931295 |
C57Bl/6 TG Mouse Lung, Dose 40ppm, 1day, biological replicate 2 |
| GSM2931296 |
C57Bl/6 TG Mouse Lung, Dose 40ppm, 1day, biological replicate 3 |
| GSM2931297 |
C57Bl/6 TG Mouse Lung, Dose 40ppm, 1day, biological replicate 4 |
| GSM2931298 |
C57Bl/6 TG Mouse Lung, Dose 40ppm, 1day, biological replicate 5 |
| GSM2931299 |
C57Bl/6 TG Mouse Lung, Dose 120ppm, 1day, biological replicate 1 |
| GSM2931300 |
C57Bl/6 TG Mouse Lung, Dose 120ppm, 1day, biological replicate 2 |
| GSM2931301 |
C57Bl/6 TG Mouse Lung, Dose 120ppm, 1day, biological replicate 3 |
| GSM2931302 |
C57Bl/6 TG Mouse Lung, Dose 120ppm, 1day, biological replicate 4 |
| GSM2931303 |
C57Bl/6 TG Mouse Lung, Dose 120ppm, 1day, biological replicate 5 |
| GSM2931304 |
C57Bl/6 WT Mouse Lung, Dose 0, 1day, biological replicate 1 |
| GSM2931305 |
C57Bl/6 WT Mouse Lung, Dose 0, 1day, biological replicate 2 |
| GSM2931306 |
C57Bl/6 WT Mouse Lung, Dose 0, 1day, biological replicate 3 |
| GSM2931307 |
C57Bl/6 WT Mouse Lung, Dose 0, 1day, biological replicate 4 |
| GSM2931308 |
C57Bl/6 WT Mouse Lung, Dose 0, 1day, biological replicate 5 |
| GSM2931309 |
C57Bl/6 WT Mouse Lung, Dose 10ppm, 1day, biological replicate 1 |
| GSM2931310 |
C57Bl/6 WT Mouse Lung, Dose 10ppm, 1day, biological replicate 2 |
| GSM2931311 |
C57Bl/6 WT Mouse Lung, Dose 10ppm, 1day, biological replicate 3 |
| GSM2931312 |
C57Bl/6 WT Mouse Lung, Dose 10ppm, 1day, biological replicate 4 |
| GSM2931313 |
C57Bl/6 WT Mouse Lung, Dose 10ppm, 1day, biological replicate 5 |
| GSM2931314 |
C57Bl/6 WT Mouse Lung, Dose 40ppm, 1day, biological replicate 1 |
| GSM2931315 |
C57Bl/6 WT Mouse Lung, Dose 40ppm, 1day, biological replicate 2 |
| GSM2931316 |
C57Bl/6 WT Mouse Lung, Dose 40ppm, 1day, biological replicate 3 |
| GSM2931317 |
C57Bl/6 WT Mouse Lung, Dose 40ppm, 1day, biological replicate 4 |
| GSM2931318 |
C57Bl/6 WT Mouse Lung, Dose 40ppm, 1day, biological replicate 5 |
| GSM2931319 |
C57Bl/6 WT Mouse Lung, Dose 120ppm, 1day, biological replicate 1 |
| GSM2931320 |
C57Bl/6 WT Mouse Lung, Dose 120ppm, 1day, biological replicate 2 |
| GSM2931321 |
C57Bl/6 WT Mouse Lung, Dose 120ppm, 1day, biological replicate 3 |
| GSM2931322 |
C57Bl/6 WT Mouse Lung, Dose 120ppm, 1day, biological replicate 4 |
| GSM2931323 |
C57Bl/6 WT Mouse Lung, Dose 120ppm, 1day, biological replicate 5 |
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| Relations |
| BioProject |
PRJNA429609 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109076_RAW.tar |
168.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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