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| Status |
Public on Jan 12, 2019 |
| Title |
DNA IP for 5hmC modified CpGs in the liver of control and HFD fed mouse liver. |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We carry out 5-hydroxymethylation DNA immunoprecipitation (hmeDIP) prior to sequencing Ion Proton P1 to report on the genome-wide 5hmC patterns of hepatic in murine hepatic steatosis.
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| Overall design |
C56BL/6 mice were fed control (11% calories by saturated fat, Research Diets Inc cat number D12329) or high fat (58% calories by saturated fat Research Diets Inc cat number D12331) diet for 17 weeks to induce obesity and hepatic steatosis. DNA was extracted from fresh frozen liver. We carry out 5-hydroxymethylation DNA immunoprecipitation (hmeDIP) prior to sequencing Ion Proton P1 to report locus specific changes in 5hmC induced by obesity. We demonstrate enrichment of 5hmC within gene bodies of induced genes with reciprocal loss of genic 5hmC in downregulated genes.
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| Contributor(s) |
Lyall MJ, Thomson JP, Drake AJ, Meehan RR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 12, 2018 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Marcus James Lyall |
| E-mail(s) |
mlyall@staffmail.ed.ac.uk
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| Organization name |
University of Edinburgh
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| Department |
University/British Heart Foundation Centre for Cardiovascular Science
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| Lab |
Drake Lab
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| Street address |
The Queen's Medical Research Institute, 47 Little France Crescent
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| City |
Edinburgh |
| State/province |
Lothian |
| ZIP/Postal code |
EH16 4T |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA429759 |
| SRA |
SRP129003 |
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