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| Status |
Public on May 20, 2019 |
| Title |
Gene Expression Analysis of Dual-κ B cells in Lupus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Autoreactive B cells expressing two different immunoglobulin light chains are present in elevated numbers in a subset of systemic lupus erythematosus patients and in MRL and MRL/lpr mouse models of lupus. Using RNAseq and genetic pathway analyses together with in vitro studies we demonstrate a role for TLR and IFN signaling in MRL/lpr dual-κ B cell expansion and/or activation. Dual-κ cells also showed increased expression of co-receptors used for T cell cognate interaction as well as enrichment of pathways involved in their signaling. Overall, our studies identify molecular pathways involved in the enrichment of dual-reactive effector B cells and reveal potential targets for their selective ablation in autoimmunity.
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| Overall design |
Examination of sorted single and dual-κ B cells from either the marginal zone or the follicles in the spleen of MRL/lpr lupus-prone mice.
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| Contributor(s) |
Sang A, Pelanda R, Danhorn T, O'Connor BP, Leach SM, Torres RM |
| Citation(s) |
30266981 |
| Submission date |
Jan 12, 2018 |
| Last update date |
May 21, 2019 |
| Contact name |
Roberta Pelanda |
| E-mail(s) |
Roberta.Pelanda@cuanschutz.edu
|
| Phone |
303-724-8666
|
| Organization name |
University of Colorado Anschutz Medical Campus
|
| Department |
Immunology&Microbiology
|
| Street address |
12800 East 19th Avenue
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA429777 |
| SRA |
SRP129011 |
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