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Status |
Public on May 22, 2021 |
Title |
ChIP-Seq of three H3 Acetylation epitopes in cultured CD8+ T cells from mice and treated with either IL2, IL2/SIINFEKL(TCR), IL2/IL4, or IL2/IL4/SIINFEKL(TCR) |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The plasticity of CD8 cells lend itself well to understanding underlying molecular mechanism for cell identity and downstream effects of microenvironment exposures. CD8 T cells in the context of memory has been studied at the epigenomic level; however, responses after immunologic microenvironment exposures culminating in a final receptor stimulation readout has not been studies in detail. We use RNA-seq to explore these questions.
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Overall design |
ChIP-Sequencing for Acetylation of Histone H3 of activated (SIINFEKL) and unactivated CD8+ T cells purified from OT1 Mouse spleens and cultured in the presence of IL-2 or IL-2/IL-4 for several days.
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Contributor(s) |
O'Connor BP, Harmacek LD, Danhorn T, Leach SM, Gelfand EW, Schedel M, Jia Y |
Citation missing |
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Submission date |
Jan 12, 2018 |
Last update date |
May 23, 2021 |
Contact name |
Brian Patrick OConnor |
E-mail(s) |
oconnorb@njhealth.org
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Organization name |
National Jewish Health
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Department |
Center for Genes, Environment and Health
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Lab |
OConnor
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Street address |
1400 Jackson St
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City |
Denver |
State/province |
Colorado |
ZIP/Postal code |
80206 |
Country |
USA |
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Platforms (1) |
GPL18635 |
Ion Torrent Proton (Mus musculus) |
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Samples (24)
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This SubSeries is part of SuperSeries: |
GSE109157 |
Molecular profiling of cultured CD8+ T cells from mice and treated with either IL2, IL2/SIIKFEKL(TCR), IL2/IL4, or IL2/IL4/SIINFEKL(TCR) |
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Relations |
BioProject |
PRJNA429801 |
SRA |
SRP129023 |