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| Status |
Public on Jan 20, 2018 |
| Title |
Expression data from MCF7 wildtype and MCF7 MDMXC463A/WT cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MDMX C463A mutation disrupts its binding with MDM2, and leads insufficient of MDM2 E3 ligase activity. With that, p53 degradation would be slower. In this case, p53 remaining its tumor suppressor function without transactivation.
To figure out the whole map of transcripts change in MDMX C463A mutation cells. We used MCF7 parental cells as control group, and MCF7 MDMXC463A/WT mutation cells as experimental group for gene chip analysis.
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| Overall design |
Triplicates of each group were cultured until log phase and subjected to RNeasy MinElute Cleanup kit (QIAGEN). Purified RNA were send to Danar-Faber core for gene-chip analysis with HTA 2.0 chip (Affymetrix).
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| Contributor(s) |
Yuan Z, Zeng W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 19, 2018 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Zhi-Min Yuan |
| E-mail(s) |
zyuan@hsph.harvard.edu
|
| Phone |
6174322139
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| Organization name |
Harvard University
|
| Department |
School of Public Health
|
| Lab |
John B. Little Center
|
| Street address |
Goldenson Building, Long wood Ave.
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02120 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE109482 |
Expression data and ChIP-seq from MCF7 wildtype and MCF7 MDMXC463A/WT cells |
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| Relations |
| BioProject |
PRJNA430812 |
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