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| Status |
Public on Jul 26, 2018 |
| Title |
Expression data from MCF-7 breast cancer cells grown in 2D and 3D |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We used a microarray to examine the global gene expression profile of MCF7 cells grown in 2D and 3D culture conditions. Our goal was to identify changes in the expression of genes that regulate iron metabolism when cellular spatial organization was altered.
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| Overall design |
MCF-7 cells were grown as 2D monolayers or 3D spheroids for 3 days before RNA extraction and hybridization on Affymetrix microarrays. We used three different 3D culture methods: Poly(2-hydroxyethyl methacrylate) (pHEMA) coated U-bottom 96-well plates, Ultra-Low Attachment 96-well plates (Corning), or the additional of methylcellulose as an aggregating agent to U-bottom 96-well plates
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| Contributor(s) |
Blanchette-Farra N, Lemler D, Konstorum A, Torti SV |
| Citation(s) |
29695834 |
| Submission date |
Jan 26, 2018 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Nicole Farra |
| E-mail(s) |
nblanchette@uchc.edu
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| Phone |
8606794576
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| Organization name |
University of Connecticut Health
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| Department |
Molecular Biology and Biophysics
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| Street address |
263 FARMINGTON AVE
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| City |
Farmington |
| State/province |
Connecticut |
| ZIP/Postal code |
06030 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (12)
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| GSM2957705 |
MCF-7 3D Ultra-Low Attachment, biological rep1 |
| GSM2957706 |
MCF-7 3D Ultra-Low Attachment, biological rep2 |
| GSM2957707 |
MCF-7 3D Ultra-Low Attachment, biological rep3 |
| GSM2957708 |
MCF-7 3D PolyHema, biological rep1 |
| GSM2957709 |
MCF-7 3D PolyHema, biological rep2 |
| GSM2957710 |
MCF-7 3D PolyHema, biological rep3 |
| GSM2957711 |
MCF-7 3D Methylcellulose, biological rep1 |
| GSM2957712 |
MCF-7 3D Methylcellulose, biological rep2 |
| GSM2957713 |
MCF-7 3D Methylcellulose, biological rep3 |
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| Relations |
| BioProject |
PRJNA432005 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109733_RAW.tar |
323.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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