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Series GSE109776 Query DataSets for GSE109776
Status Public on Mar 18, 2019
Title Gene expression after 1 and 5 days of cigarette smoke exposure in mice with chronically inflamed or healthy lungs
Organism Mus musculus
Experiment type Expression profiling by array
Summary These studies tested the hypotheses that smoke induces changes in mRNA profiles that are dependent on sex and the health status of the lung, and that the effects of smoke are different after 1 day compared to 5 days of smoke exposure. The ways in which the lungs modulate their response to cigarette smoke after repeated exposures are important for understanding the toxicology of smoke, for developing biomarkers of chronic smoke exposure, and for understanding the therapeutic potential in regulatory signaling pathways that are beneficial or detrimental to lung health.
Sex-matched 5-7-week old wildtype (WT) and Scnn1b-overexpressing (BENaC) littermates were exposed to cigarette smoke or sham (room air) exposure. Exposure occurred in a plexiglass chamber attached to a smoke delivery device using an exposure chamber and smoking machine (inExpose Exposure System, SCIREQ, Chandler, AZ). Mice were exposed to mainstream + sidestream smoke from 6 reference cigarettes with filters removed per day (3R4F research cigarettes, University of Kentucky). Each cigarette was puffed for 2 sec every 25 sec, using the standard Federal Trade Commission smoking machine protocol. The sham-exposed control mice were exposed to room air in the exposure chamber for a time equivalent to that needed for active smoke exposure. Mice were exposed to cigarette or sham smoke for 1 day or 5 consecutive days. Samples were harvested 4 hours after the completion of the final smoke exposure. The right lung was used for gene expression analysis.
 
Overall design Mice were exposed to cigarette smoke or sham (room air) for 1 day or 5 consecutive days. Samples were harvested 4 hours after the completion of the final smoke exposure. The right lung was used for gene expression analysis. Gene expression was measured using the Affymetrix moGene2.1 array.
 
Contributor(s) Engle ML, Dang H, Parker JS, Doerschuk CM
Citation(s) 30818335
Submission date Jan 29, 2018
Last update date Mar 18, 2019
Contact name Claire M Doerschuk
Organization name UNC Chapel Hill
Street address 125 Mason Farm Rd. CB#7248
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platforms (1)
GPL22070 [MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript-level na35ens81]
Samples (79)
GSM2967093 BENaC_Smoke_Female_1day_Rep1
GSM2967094 BENaC_Smoke_Female_1day_Rep2
GSM2967095 BENaC_Smoke_Female_1day_Rep3
Relations
BioProject PRJNA431999

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109776_RAW.tar 377.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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