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| Status |
Public on Jan 30, 2018 |
| Title |
Role of skeletal muscle in lung development. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Skeletal (striated) muscle is one of the four basic tissue types, together with the epithelium, connective and nervous tissues. Lungs, on the other hand, develop from the foregut and among various cell types contain smooth, but not skeletal muscle. Therefore, during earlier stages of development, it is unlikely that skeletal muscle and lung depend on each other. However, during the later stages of development, respiratory muscle, primarily the diaphragm and the intercostal muscles, execute so called fetal breathing-like movements (FBMs), that are essential for lung growth and cell differentiation. In fact, the absence of FBMs results in pulmonary hypoplasia, the most common cause of death in the first week of human neonatal life. Most knowledge on this topic arises from in vivo experiments on larger animals and from various in vitro experiments. In the current era of mouse mutagenesis and functional genomics, it was our goal to develop a mouse model for pulmonary hypoplasia.
We employed various genetically engineered mice lacking different groups of respiratory muscles or lacking all the skeletal muscle and established the criteria for pulmonary hypoplasia in mice, and therefore established a mouse model for this disease. We followed up this discovery with systematic subtractive microarray analysis approach and revealed novel functions in lung development and disease for several molecules. We believe that our approach combines elements of both in vivo and in vitro approaches and allows us to study the function of a series of molecules in the context of lung development and disease and, simultaneously, in the context of lung's dependence on skeletal muscle-executed FBMs.
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| Overall design |
Mouse embryos were obtained at E18.5 for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Baguma-Nibasheka M, Gugic D, Saraga-Babic M, Kablar B |
| Citation(s) |
22648538 |
| Submission date |
Jan 29, 2018 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Boris Kablar |
| E-mail(s) |
Boris.Kablar@Dal.Ca
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| Phone |
(902) 494-3169
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| Organization name |
Dalhousie University
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| Department |
Medical Neuroscience
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| Street address |
5850 College Street
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| City |
Halifax |
| State/province |
Nova Scotia |
| ZIP/Postal code |
B3H 4R2 |
| Country |
Canada |
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| Platforms (2) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
| GPL340 |
[MOE430B] Affymetrix Mouse Expression 430B Array |
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| Samples (4)
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| GSM2967236 |
E18.5 Myf5-/-:MyoD-/- mouse lung, MOE430B |
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| Relations |
| BioProject |
PRJNA432025 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109783_RAW.tar |
36.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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