 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 30, 2021 |
| Title |
Expression data from kidney tissues treated with vehicle, losartan and metformin |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Chronic kidney disease (CKD) is one of the major global health problems with high incidence, poor prognosis and high medical cost. However, few pharmacological options are available for CKD. Metformin is widely used for treatment of type-2 diabetes, but recent works showed that metformin ameliorates tumor progression, inflammatory disease and tissue fibrosis. Whether metformin ameliorates non-diabetic chronic glomerular disease and CKD is unexplored. Here we showed that metformin or losartan (used as control) has protective effects against CKD by suppressing proteinuria, renal inflammation, fibrosis and glomerular injury in Alport syndrome mouse model, which spontaneously manifests chronic glomerular and kidney disease. Transcriptome analysis showed that metformin and losartan influenced molecular pathways of metabolism and inflammation, respectively. While metformin specifically affected genes that were classified as metabolic regulators, losartan specifically altered genes that were classified as inflammatory regulators. Metformin also induced multiple signaling pathways not affected by losartan. Overall, metformin ameliorates non-diabetic chronic glomerular diseases, and could be considered a therapeutic option for CKD.
We used microarrays to investigate the global gene expression underlying the protective effects of metformin on Alport syndrome mice model
|
| |
|
| Overall design |
Total RNA were extracted from kidney tissues of wildtype and vehicle-treated Alport mice (Alport CON), losartan-treated Alport mice (Alport Losartan), metformin-treated Alport mice (Alport metformin). Extracted RNA samples were hybridized to Affymetrix Clariom S Arrays.
|
| |
|
| Contributor(s) |
Kai H, Omachi K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 30, 2018 |
| Last update date |
Jan 31, 2021 |
| Contact name |
Kohei Omachi |
| E-mail(s) |
kohei.o.0420@gmail.com
|
| Phone |
81963714407
|
| Organization name |
Kumamoto University
|
| Department |
Molecular Medicine
|
| Lab |
Molecular Medicine (PI: H Kai)
|
| Street address |
5-1, Oe-honmachi
|
| City |
Kumammoto |
| ZIP/Postal code |
8620973 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
|
| Samples (12)
|
| GSM2971587 |
wild type treated with vehicle replicate 1 |
| GSM2971588 |
wild type treated with vehicle replicate 2 |
| GSM2971589 |
wild type treated with vehicle replicate 3 |
| GSM2971590 |
Alport treated with vehicle replicate 1 |
| GSM2971591 |
Alport treated with vehicle replicate 2 |
| GSM2971592 |
Alport treated with vehicle replicate 3 |
| GSM2971593 |
Alport treated with losartan replicate 1 |
| GSM2971594 |
Alport treated with losartan replicate 2 |
| GSM2971595 |
Alport treated with losartan replicate 3 |
| GSM2971596 |
Alport treated with metformin replicate 1 |
| GSM2971597 |
Alport treated with metformin replicate 2 |
| GSM2971598 |
Alport treated with metformin replicate 3 |
|
| Relations |
| BioProject |
PRJNA432213 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109861_RAW.tar |
16.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...