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Series GSE110053 Query DataSets for GSE110053
Status Public on May 22, 2018
Title A map of the PGC-1α- and NT-PGC-1α-regulated transcriptional network in brown adipose tissue [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Transcriptional coactivator PGC-1α and its splice variant NT-PGC-1α play crucial roles in regulating cold-induced thermogenesis in brown adipose tissue (BAT). PGC-1α and NT-PGC-1α are highly induced by cold in BAT and subsequently bind to and coactivate many different transcription factors to regulate expression of genes involved in mitochondrial biogenesis, fatty acid oxidation, respiration and thermogenesis. To identify the complete repertoire of PGC-1α and NT-PGC-1α target genes in BAT, we analyzed genome-wide DNA-binding and gene expression profiles. We find that PGC-1α-/NT-PGC-1α binding broadly associates with cold-mediated transcriptional activation. In addition to their known target genes in mitochondrial biogenesis, fatty acid oxidation, respiration and thermogenesis, PGC-1α and NT-PGC-1α target to a broad spectrum of genes involved in diverse biological pathways including ubiquitin-dependent protein catabolism, ribonucleoprotein complex biosynthesis, phospholipid biosynthesis, angiogenesis, glycogen metabolism, phosphorylation, and autophagy. Our findings expand the number of genes and biological pathways that may be regulated by PGC-1α and NT-PGC-1α and provide further insight into the transcriptional regulatory network in which PGC-1α and NT-PGC-1α coordinate a comprehensive transcriptional response in BAT in response to cold.
Overall design Examination of genome-wide PGC-1α/NT-PGC-1α binding sites in brown adipose tissue
Contributor(s) Chang J, Ghosh S, Newman S, Salbaum M
Citation(s) 29777200
Submission date Feb 02, 2018
Last update date May 22, 2018
Contact name Ji Suk Chang
Organization name Pennington Biomedical Research Center
Department Gene Regulation and Metabolism
Street address 6400 Perkins Rd.
City Baton Rouge
State/province LA
ZIP/Postal code 70808
Country USA
Platforms (1)
GPL15907 AB 5500xl Genetic Analyzer (Mus musculus)
Samples (4)
GSM2977052 WT1_ChIP
GSM2977053 WT1_Input
GSM2977054 WT2_ChIP
This SubSeries is part of SuperSeries:
GSE110056 A map of the PGC-1α- and NT-PGC-1α-regulated transcriptional network in brown adipose tissue
BioProject PRJNA432780
SRA SRP132120

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Supplementary file Size Download File type/resource
GSE110053_Galaxy29-_Genome_Coverage_mm10_and_WT1-2_.bedgraph.gz 32.5 Kb (ftp)(http) BEDGRAPH
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