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Series GSE110055 Query DataSets for GSE110055
Status Public on May 22, 2018
Title A map of the PGC-1α- and NT-PGC-1α-regulated transcriptional network in brown adipose tissue [SAGE]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Transcriptional coactivator PGC-1α and its splice variant NT-PGC-1α play crucial roles in regulating cold-induced thermogenesis in brown adipose tissue (BAT). PGC-1α and NT-PGC-1α are highly induced by cold in BAT and subsequently bind to and coactivate many different transcription factors to regulate expression of genes involved in mitochondrial biogenesis, fatty acid oxidation, respiration and thermogenesis. To identify the complete repertoire of PGC-1α and NT-PGC-1α target genes in BAT, we analyzed genome-wide DNA-binding and gene expression profiles. We find that PGC-1α-/NT-PGC-1α binding broadly associates with cold-mediated transcriptional activation. In addition to their known target genes in mitochondrial biogenesis, fatty acid oxidation, respiration and thermogenesis, PGC-1α and NT-PGC-1α target to a broad spectrum of genes involved in diverse biological pathways including ubiquitin-dependent protein catabolism, ribonucleoprotein complex biosynthesis, phospholipid biosynthesis, angiogenesis, glycogen metabolism, phosphorylation, and autophagy. Our findings expand the number of genes and biological pathways that may be regulated by PGC-1α and NT-PGC-1α and provide further insight into the transcriptional regulatory network in which PGC-1α and NT-PGC-1α coordinate a comprehensive transcriptional response in BAT in response to cold.
Overall design Examination of brown adipose tissue mRNA profiles of mice housed at 28°C or exposed to 4°C for 6h
Contributor(s) Chang J, Ghosh S, Newman S, Salbaum M
Citation(s) 29777200
Submission date Feb 02, 2018
Last update date May 22, 2018
Contact name Ji Suk Chang
Organization name Pennington Biomedical Research Center
Department Gene Regulation and Metabolism
Street address 6400 Perkins Rd.
City Baton Rouge
State/province LA
ZIP/Postal code 70808
Country USA
Platforms (1)
GPL15907 AB 5500xl Genetic Analyzer (Mus musculus)
Samples (12)
GSM2977066 WT_28C-9
GSM2977067 WT_28C-8
GSM2977068 WT_28C-7
This SubSeries is part of SuperSeries:
GSE110056 A map of the PGC-1α- and NT-PGC-1α-regulated transcriptional network in brown adipose tissue
BioProject PRJNA432781
SRA SRP132122

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Supplementary file Size Download File type/resource
GSE110055_Chang_SAGE_09.2013_DESeqNorm.txt.gz 539.7 Kb (ftp)(http) TXT
GSE110055_Chang_SAGE_09.2013_rawcounts.txt.gz 307.4 Kb (ftp)(http) TXT
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