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| Status |
Public on Feb 06, 2019 |
| Title |
IFNb/LPS activates convergence of inflammatory gene expression patterns between adipocytes and myeloid cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We examined the transciptomic changes to primary adipocytes resulting from exposure to IFNb and/or LPS, in order to define the mechanisms driving adipocyte inflammatory capacity and resulting transcriptomic convergence with iinflammatory myeloid cells.
Inguinal adipose tissue was obtained from 8 weeks old aged male C57BL/6 mice and stromal vascular fraction was maximally differentiated into primary adipocytes. Cultured cells were treated with IFNb (200U/ml) or saline for 3 hours and thereafter with LPS (100ng/ml) or saline for 4 hours and then submitted for RNA-sequencing.
Results: We mapped approximately 20 million reads per sample to the mm10 genome, and analyzed all transcripts with >3 reads in 100% of at least one experimental condition.
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|
| Overall design |
Our study characterizes the transcriptional changes induced by IFNb, LPS and IFNb+LPS through RNAsequencing of adipocytes. Our results identify that IFNb unlocks a convergence in gene expression patterns between adipocytes and myeloid cells. Further, IFNb priming augments LPS-induced pathways in adipocytes.
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| |
|
| Contributor(s) |
Divanovic S, Karns R, Chan C |
| Citation(s) |
32488081 |
| Submission date |
Feb 06, 2018 |
| Last update date |
May 23, 2021 |
| Contact name |
Rebekah Karns |
| E-mail(s) |
Rebekah.Karns@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Gastroenterology, Hepatology, & Nutrition
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45276 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (8)
|
| GSM2983061 |
Primary adipocytes treated with Saline 1 |
| GSM2983062 |
Primary adipocytes treated with Saline 2 |
| GSM2983063 |
Primary adipocytes treated with IFNb 1 |
| GSM2983064 |
Primary adipocytes treated with IFNb 2 |
| GSM2983065 |
Primary adipocytes treated with LPS 1 |
| GSM2983066 |
Primary adipocytes treated with LPS 2 |
| GSM2983067 |
Primary adipocytes treated with LPS+IFNb 1 |
| GSM2983068 |
Primary adipocytes treated with LPS+IFNb 2 |
|
| Relations |
| BioProject |
PRJNA433230 |
| SRA |
SRP132305 |
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