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| Status |
Public on Apr 03, 2018 |
| Title |
Epigenetic profiling of OVA-specific CD8+ TILs from mice treated with control, anti-B7S1, anti-PD-1 or anti-B7S1+anti-PD-1 antibodies |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Combinational blockade of B7S1 and PD-1 exhibits stronger anti-tumor efficacy than monotherapies. In order to further understand the mechanisms whereby blockade of B7S1 or PD-1 inhibits tumor growth, we conducted ATAC-seq analysis of OVA-specific CD8+ TILs from E.G7-bearing mice treated with control antibodies, anti-B7S1, anti-PD-1 monotherapy or combinational therapy.
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| Overall design |
1 million E.G7 were inoculated subcutaneously into mice on Day 0. On Day 21, OVA-specific CD8+ TILs from E.G7-bearing mice treated with control, anti-B7S1, anti-PD-1 or anti-B7S1+anti-PD-1 antibodies(6 mice per group) were sorted using BD FACSAria Cell Sorter. The collected cells were then subjected to construction of library for ATAC-seq.
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| Contributor(s) |
Li J, Li Y |
| Citation(s) |
29625896 |
| Submission date |
Feb 07, 2018 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Chen Dong |
| Organization name |
Tsinghua University
|
| Department |
Institute for Immunology and School of Medicine
|
| Lab |
Dongchen's lab
|
| Street address |
Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA433479 |
| SRA |
SRP132461 |
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