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| Status |
Public on Jan 30, 2019 |
| Title |
Transcriptomic analysis of active form of Srebf1-overexpressed fibroblasts in bleomycin-induced lung fibrosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pulmonary fibrosis (PF) is an intractable disorder with a poor prognosis. Although lung fibroblasts play central roles in PF, their key regulatory molecules remain unclear. We performed transcriptome analysis of lung fibroblasts from bleomycin- and silica-treated murine lungs and identified 55 hub transcription factors highly connected to gene modules differentially expressed in PF. To elucidate whether fibroblast-specific intervention against the hub transcription factor Srebf1 modulates pathogenic activation of lung fibroblasts in vivo, we intratracheally-transferred active form of Srebf1-overexpressed fibroblasts into bleomycin-treated lungs and performed global transcriptome analysis.
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| Overall design |
Active form of Srebf1-overexpressed fibroblasts and mock-expressed fibroblasts were intratracheally-transferred to B6 mice at day 5 post-administration of 2 mg/kg of bleomycin. Expression of active form of Srebf1 was induced by doxycycline administration at day 7 post-administration of 2 mg/kg of bleomycin. Donor cells were recovered at day 10 post--administration of 2 mg/kg of bleomycin by cell sorting. Global transcriptome of transferred fibroblasts was generated by 3'SAGE-seq using Ion Proton sequencer.
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| Contributor(s) |
Shichino S, Hashimoto S, Otsuji M, Deshimaru S, Ueha S, Matsushima K |
| Citation(s) |
30626759, 34014607, 34759926, 35780190 |
| Submission date |
Feb 13, 2018 |
| Last update date |
Jul 14, 2022 |
| Contact name |
Shigeyuki Shichino |
| E-mail(s) |
s_shichino@rs.tus.ac.jp
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| Organization name |
Tokyo University of Science
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| Department |
Department of Molecular regulation of Inflammatory and Immune Diseases
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| Street address |
2641, Yamasaki, Noda-shi
|
| City |
Chiba |
| ZIP/Postal code |
278-0022 |
| Country |
Japan |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE110711 |
Transcriptome network analysis reveals the LXR-Srebf1 axis as protective hub of fibroblast activation in murine pulmonary fibrosis |
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| Relations |
| BioProject |
PRJNA433942 |
| SRA |
SRP132784 |
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