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| Status |
Public on May 01, 2018 |
| Title |
Toxic effects and foundation of proton radiation on the early-life stage of zebrafish development |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
Zebrafish is an ideal model for the toxicity studies on medicines and environmental genetic toxicants.Different development defects were observed in zebrafish embryos exposed to -ray and heavy ion (carbon or iron) irradiation
We used microarrays to detail the gene expression situation after radiation.
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| Overall design |
Proton beam irradiation was carried out at the terminal of the heavy ion accelerator facility at Peking University. The proton beam energy was 8 MeV/u, and the dose rate was 7.5 Gy/min.To detect the gene expression, three experimental groups were established as follows: zebrafish embryos collected from wild type breeding pairs, pooled, and allowed to develop for 24 h. One group of, 60 embryos was withdrawn to serve as the non-irradiated control (CK). To investigate the radiation response, two other groups of, 60 embryos each were withdrawn and irradiated with 12 (IR1) or 15 Gy (IR2) of protons. Embryos in all three groups were then allowed to grow and develop at 28.5 ◦C using standard maintenance protocols.
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| Contributor(s) |
Zhao B, Li X, Zha X |
| Citation missing |
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| Submission date |
Feb 13, 2018 |
| Last update date |
May 02, 2018 |
| Contact name |
Baoquan Zhao |
| Organization name |
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences
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| Street address |
Taiping road,27
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| City |
Beijing |
| ZIP/Postal code |
100850 |
| Country |
China |
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| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
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| Samples (5)
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| GSM3003559 |
larvae unirradiated_CK, biological rep1 |
| GSM3003560 |
larvae irradiated with 12Gy_IR1, biological rep1 |
| GSM3003561 |
larvae irradiated with 15Gy_IR2, biological rep1 |
| GSM3003562 |
larvae irradiated with 15Gy_IR2, biological rep2 |
| GSM3003563 |
larvae irradiated with 15Gy_IR2, biological rep3 |
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| Relations |
| BioProject |
PRJNA434025 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE110583_RAW.tar |
9.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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