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Series GSE110583 Query DataSets for GSE110583
Status Public on May 01, 2018
Title Toxic effects and foundation of proton radiation on the early-life stage of zebrafish development
Organism Danio rerio
Experiment type Expression profiling by array
Summary Zebrafish is an ideal model for the toxicity studies on medicines and environmental genetic toxicants.Different development defects were observed in zebrafish embryos exposed to -ray and heavy ion (carbon or iron) irradiation
We used microarrays to detail the gene expression situation after radiation.
 
Overall design Proton beam irradiation was carried out at the terminal of the heavy ion accelerator facility at Peking University. The proton beam energy was 8 MeV/u, and the dose rate was 7.5 Gy/min.To detect the gene expression, three experimental groups were established as follows: zebrafish embryos collected from wild type breeding pairs, pooled, and allowed to develop for 24 h. One group of, 60 embryos was withdrawn to serve as the non-irradiated control (CK). To investigate the radiation response, two other groups of, 60 embryos each were withdrawn and irradiated with 12 (IR1) or 15 Gy (IR2) of protons. Embryos in all three groups were then allowed to grow and develop at 28.5 ◦C using standard maintenance protocols.
 
Contributor(s) Zhao B, Li X, Zha X
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Submission date Feb 13, 2018
Last update date May 02, 2018
Contact name Baoquan Zhao
Organization name Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences
Street address Taiping road,27
City Beijing
ZIP/Postal code 100850
Country China
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (5)
GSM3003559 larvae unirradiated_CK, biological rep1
GSM3003560 larvae irradiated with 12Gy_IR1, biological rep1
GSM3003561 larvae irradiated with 15Gy_IR2, biological rep1
Relations
BioProject PRJNA434025

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE110583_RAW.tar 9.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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