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| Status |
Public on Dec 31, 2018 |
| Title |
Effect of loss of ciliogenesis on myoblast quiescence |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Quiescence is an actively maintained state of the cell cycle;aberrations in which can result in severe consequencces for development and tissue homeostasis. Primary cilia are signaling centres derived from the centrosome which are associated with quiescence.Using a mouse skeletal myoblast cell culture system that can be induced to exit the cell cycle reversibly into quiescence or irreversibly into differentiation, we studied the effect of loss of ciliogenesis on quiescence.
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| Overall design |
We performed microarray analysisis to study changes in the global transcriptome of IFT88KD myoblasts in different cell cycle states. RNA was harvested from C2C12 myoblasts that underwent siRNA treatment using either IFT88 or negative control siRNA at four different cell cycle states- Proliferating (MB), Quiescent (G0), Reentry into cell cycle for 2 hours (R2), and 48 hours into terminal differentiation (D48). RNA was converted to cDNA, labelled and used to probe Affymetrix mouse 420A 2.0 arrays.
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| Contributor(s) |
Venugopal N, Gala HP, Dhawan J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 16, 2018 |
| Last update date |
Jan 01, 2019 |
| Contact name |
Nisha Venugopal |
| Organization name |
CCMB
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| Street address |
Habsiguda, Uppal Road
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| City |
Hyderabad |
| State/province |
Telangana |
| ZIP/Postal code |
500007 |
| Country |
India |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (24)
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| GSM3015732 |
IFT88KD Myoblasts,Proliferating condition,Replicate1 |
| GSM3015733 |
IFT88KD Myoblasts,Proliferating condition,Replicate2 |
| GSM3015734 |
IFT88KD Myoblasts,Proliferating condition,Replicate3 |
| GSM3015735 |
Control Myoblasts,Proliferating condition,Replicate1 |
| GSM3015736 |
Control Myoblasts,Proliferating condition,Replicate2 |
| GSM3015737 |
Control Myoblasts,Proliferating condition,Replicate3 |
| GSM3015738 |
IFT88KD Myoblasts,Quiescence conditions,Replicate1 |
| GSM3015739 |
IFT88KD Myoblasts,Quiescence conditions,Replicate2 |
| GSM3015740 |
IFT88KD Myoblasts,Quiescence conditions,Replicate3 |
| GSM3015741 |
Control Myoblasts,Quiescence conditions,Replicate1 |
| GSM3015742 |
Control Myoblasts,Quiescence conditions,Replicate2 |
| GSM3015743 |
Control Myoblasts,Quiescence conditions,Replicate3 |
| GSM3015744 |
IFT88KD Myoblasts,R2 hours,Replicate1 |
| GSM3015745 |
IFT88KD Myoblasts,R2 hours,Replicate2 |
| GSM3015746 |
IFT88KD Myoblasts,R2 hours,Replicate3 |
| GSM3015747 |
Control Myoblasts,R2 hours,Replicate1 |
| GSM3015748 |
Control Myoblasts,R2 hours,Replicate2 |
| GSM3015749 |
Control Myoblasts,R2 hours,Replicate3 |
| GSM3015750 |
IFT88KD Myoblasts,D48 hours,Replicate1 |
| GSM3015751 |
IFT88KD Myoblasts,D48 hours,Replicate2 |
| GSM3015752 |
IFT88KD Myoblasts,D48 hours,Replicate3 |
| GSM3015753 |
Control Myoblasts,D48 hours,Replicate1 |
| GSM3015754 |
Control Myoblasts,D48 hours,Replicate2 |
| GSM3015755 |
Control Myoblasts,D48 hours,Replicate3 |
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| Relations |
| BioProject |
PRJNA434444 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE110742_RAW.tar |
36.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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