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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 21, 2020 |
| Title |
Expression data from spleen-derived and tumor-induced CD11b+ MDSC |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
CD11b+ myeloid-derived suppressor cells (MDSCs) have been shown to become activated at the tumor site and acquired a more suppressive phenotype compared to the splenic counterpart. This activated status promotes cancer progression by creating an immune suppressive niche around the tumor, by providing nourishment to the neoplastic cells, and by promoting metastases. To start understanding the differences between splenic and tumor infiltrating CD11b, genome-wide transcriptome analysis was performed.
We used gene expression analysis to identify those factors, secreted by tumor-infiltrating MDSC, which could drive emathopoiesis. Moreover, we compare gene expression profile of tumor-induced MDSC, obtained from either the spleen or the tumor infiltrate of tumor-bearing mice.
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| Overall design |
CD11b+ cells were immunomagnetically enriched from various murine tissues and experimental conditions. cRNA samples were prepared according to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004, and hybridized to the Affymetrix GeneChip MOE430 2.0 array which contains more than 45,000 probe sets, representing more than 34,000 genes. CD11b+ cells obtained from the spleen of healthy BALB/c mice were used as reference sample for tumor induced CD11b+ MDSC, enriched from either the spleen or the tumor infiltrate of tumor-bearing mice.
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| Contributor(s) |
Bicciato S, Serafini P |
| Citation(s) |
32554710 |
| Submission date |
Feb 18, 2018 |
| Last update date |
Jun 23, 2020 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
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| Phone |
+39-049-827-6108
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| Organization name |
University of Padova
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| Department |
Molecular Medicine
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| Street address |
via U. Bassi 59/b
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL23097 |
[Mouse4302_Mm_EntrezG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Mouse4302_Mm_ENTREZG_21.0.0] |
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| Samples (12)
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| GSM3016437 |
CD11b+ MDSC from spleen, biological rep1 |
| GSM3016438 |
CD11b+ MDSC from spleen, biological rep2 |
| GSM3016439 |
CD11b+ MDSC from spleen, biological rep3 |
| GSM3016440 |
CD11b+ MDSC from spleen, biological rep4 |
| GSM3016441 |
CD11b+ MDSC from spleen, biological rep5 |
| GSM3016442 |
CD11b+ MDSC from spleen, biological rep6 |
| GSM3016443 |
CD11b+ MDSC from tumor, biological rep1 |
| GSM3016444 |
CD11b+ MDSC from tumor, biological rep2 |
| GSM3016445 |
CD11b+ MDSC from tumor, biological rep3 |
| GSM3016446 |
CD11b+ MDSC from tumor, biological rep4 |
| GSM3016447 |
CD11b+ MDSC from tumor, biological rep5 |
| GSM3016448 |
CD11b+ MDSC from tumor, biological rep6 |
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| This SubSeries is part of SuperSeries: |
| GSE110774 |
Expression data from myeloid-derived suppressor cells (MDSCs) |
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| Relations |
| BioProject |
PRJNA434517 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE110772_RAW.tar |
55.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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