|
| Status |
Public on Mar 19, 2019 |
| Title |
Chromatin accessibility in C. albicans stimulated HoxER-PU.1 WT and HoxER-PU.1 KO neutrophils and DMSO, TSA or Entinostat treated HoxER-PU.1 WT neutrophils |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Neutrophils are essential first line defense cells against invading pathogens, yet their inappropriate activation contributes to immunological diseases and can cause collateral tissue damage. However, if and how neutrophils cell-intrinsically titrate their inflammatory response remains unknown. Here, we conditionally deleted PU.1, a key myeloid transcription factor, from the neutrophils of mice undergoing fungal infection, and then performed comprehensive epigenomic profiling. We find that a major function of PU.1 is to restrain the neutrophils’ immune response by broadly suppressing genomic enhancer outputs via recruiting histone deacetylase activity, thereby limiting the immune-stimulatory AP1-transcription factor JUNB from entering chromatin. Thus, neutrophils rely on a direct PU.1 repressor function as rheostat of the inflammatory chromatin state, safeguarding their epigenome from undergoing uncontrolled activation prior to pathogenic stimulation.
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| |
|
| Overall design |
Tn-Seq profiles of 2 hours C. albicans stimulated wildtype and PU.1 deleted neutrophils as well as DMSO, TSA or Entinostat treated wildtype neutrophils were generated by deep sequencing in duplicates.
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| |
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| Contributor(s) |
Rosenbauer F, Fischer J, Walter C, Tönges A |
| Citation(s) |
30911105 |
| Submission date |
Feb 20, 2018 |
| Last update date |
Oct 28, 2021 |
| Contact name |
Frank Rosenbauer |
| E-mail(s) |
frank.rosenbauer@ukmuenster.de
|
| Organization name |
University Hospital Münster
|
| Department |
Institute of Molecular Tumor Biology
|
| Street address |
Robert-Koch-Str. 43
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (13)
|
|
| This SubSeries is part of SuperSeries: |
| GSE110865 |
Safeguard function of PU.1 shapes the inflammatory epigenome of neutrophils |
|
| Relations |
| BioProject |
PRJNA434709 |
| SRA |
SRP133138 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE110860_ATAC_ffU_peaks.bed.gz |
482.5 Kb |
(ftp)(http) |
BED |
| GSE110860_ATAC_wtU-wtCa_wtCa_spec_peaks.bed.gz |
15.2 Kb |
(ftp)(http) |
BED |
| GSE110860_ATAC_wtU-wtCa_wtU_spec_peaks.bed.gz |
101 b |
(ftp)(http) |
BED |
| GSE110860_ATAC_wtU_peaks.bed.gz |
298.1 Kb |
(ftp)(http) |
BED |
| GSE110860_DMSO_rep1_rmdup_cutSites.wig.gz |
199.4 Mb |
(ftp)(http) |
WIG |
| GSE110860_DMSO_rep2_rmdup_cutSites.wig.gz |
216.8 Mb |
(ftp)(http) |
WIG |
| GSE110860_Enti_rep1_rmdup_cutSites.wig.gz |
191.3 Mb |
(ftp)(http) |
WIG |
| GSE110860_Enti_rep2_rmdup_cutSites.wig.gz |
171.4 Mb |
(ftp)(http) |
WIG |
| GSE110860_TSA_rmdup_cutSites.wig.gz |
161.5 Mb |
(ftp)(http) |
WIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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