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| Status |
Public on Feb 21, 2020 |
| Title |
Transcriptomic analysis of tumor-infiltrating dendritic cells in mouse subcutaneous tumor models |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CCR7+ tumor-infiltrating DCs (tDCs) transport tumor antigens from the tumor to the draining lymph node (dLN), and thus play a pivotal role in anti-tumor CD8+ T cell responses. However, the molecular mechanisms that underlie tDC activity remain poorly understood. To elucidate this problem, we investigated the transcriptomic properties of CCR7+ tDCs in mouse subcutaneous tumor models.
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| Overall design |
In the LLC and B16F10 subcutaneous tumor models, tumor-infiltrating lineage-negative (CD3, CD19, NK1.1, Ly-6G, Ter-119)- Ly-6C- MHC class II+ CCR7+ tDCs, lineage- CCR7- MHC class II+ cells and CD326- migratory DCs in the dLN were purified by cell sorting at day 10 or day 14 post-tumor inoculation. Then, we performed transcriptome analysis on these cells by 3’ SAGE-seq.
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| Contributor(s) |
Shichino S, Ueha S, Deshimaru S, Ogiwara H, Kurachi M, Abe J, Hashimoto S, Matsushima K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 21, 2018 |
| Last update date |
Feb 23, 2020 |
| Contact name |
Shigeyuki Shichino |
| E-mail(s) |
s_shichino@rs.tus.ac.jp
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| Organization name |
Tokyo University of Science
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| Department |
Department of Molecular regulation of Inflammatory and Immune Diseases
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| Street address |
2641, Yamasaki, Noda-shi
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| City |
Chiba |
| ZIP/Postal code |
278-0022 |
| Country |
Japan |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA435413 |
| SRA |
SRP133245 |
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