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| Status |
Public on Nov 14, 2018 |
| Title |
Transcriptome analysis of fibroblast subpopulations isolated from neonatal murine dermis. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
During aging, stromal functions are thought to be impaired, yet little is known whether this stems from molecular and cellular changes of fibroblasts, a major component of stroma. Using population- and single-cell whole transcriptomics, and long-term lineage tracing, we studied alterations in murine dermal fibroblasts during physiological aging under different dietary regimes known to affect longevity. We show that the identity of aged fibroblasts becomes undefined, with the distinct fibroblast states present in young skin no longer clearly demarcated. In addition, old fibroblasts not only reduce the expression of genes involved in the formation of the extracellular matrix, but intriguingly, also gain adipogenic traits, paradoxically becoming similar to neonatal pro-adipogenic fibroblasts. These age-related alterations are sensitive to systemic changes in metabolism: long-term caloric restriction prevents them in old fibroblasts in a reversible manner, whereas a high-fat diet potentiates them in young fibroblasts. Finally, inhibition of the master regulator of adipogenesis, PPARgamma, attenuates fibroblast aging in vivo, providing potential anti-aging therapeutic alternatives to caloric restriction.
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| Overall design |
CD26+ papillary, Dlk1+ reticular, Sca1+ pre-adipogenic and Sca1+/Dlk1+ pre-adiogenic fibroblasts were FACS isolated from three newborn (P2) female littermates. N=3
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| Contributor(s) |
Salzer M, Aznar-Benitah S |
| Citation(s) |
30415840 |
| Submission date |
Feb 22, 2018 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Antoni Berenguer-LLergo |
| E-mail(s) |
aberenguerl@tauli.cat
|
| Organization name |
Parc Taulí Research and Innovation Institute (I3PT)
|
| Department |
A8G2 Group - Rheumatology Service
|
| Street address |
Parc del Taulí, 1
|
| City |
Sabadell |
| State/province |
Barcelona |
| ZIP/Postal code |
08208 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
|
| Samples (12)
|
| GSM3019659 |
CD26+ upper papillary fibroblasts from biological replicate NB1 |
| GSM3019660 |
Dlk1+ reticular fibroblasts from biological replicate NB1 |
| GSM3019661 |
Dlk1+ Sca1+ pre-adipogenic fibroblasts from biological replicate NB1 |
| GSM3019662 |
Dlk1- Sca1+ pre-adipogenic fibroblasts from biological replicate NB1 |
| GSM3019663 |
CD26+ upper papillary fibroblasts from biological replicate NB2 |
| GSM3019664 |
Dlk1+ reticular fibroblasts from biological replicate NB2 |
| GSM3019665 |
Dlk1+ Sca1+ pre-adipogenic fibroblasts from biological replicate NB2 |
| GSM3019666 |
Dlk1- Sca1+ pre-adipogenic fibroblasts from biological replicate NB2 |
| GSM3019667 |
CD26+ upper papillary fibroblasts from biological replicate NB3 |
| GSM3019668 |
Dlk1+ reticular fibroblasts from biological replicate NB3 |
| GSM3019669 |
Dlk1+ Sca1+ pre-adipogenic fibroblasts from biological replicate NB3 |
| GSM3019670 |
Dlk1- Sca1+ pre-adipogenic fibroblasts from biological replicate NB3 |
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| This SubSeries is part of SuperSeries: |
| GSE110983 |
The transcriptome of murine dermal fibroblasts |
|
| Relations |
| BioProject |
PRJNA435490 |
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