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| Status |
Public on Nov 14, 2018 |
| Title |
The effect of high fat diet on the transcriptome of murine dermal fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
During aging, stromal functions are thought to be impaired, yet little is known whether this stems from molecular and cellular changes of fibroblasts, a major component of stroma. Using population- and single-cell whole transcriptomics, and long-term lineage tracing, we studied alterations in murine dermal fibroblasts during physiological aging under different dietary regimes known to affect longevity. We show that the identity of aged fibroblasts becomes undefined, with the distinct fibroblast states present in young skin no longer clearly demarcated. In addition, old fibroblasts not only reduce the expression of genes involved in the formation of the extracellular matrix, but intriguingly, also gain adipogenic traits, paradoxically becoming similar to neonatal pro-adipogenic fibroblasts. These age-related alterations are sensitive to systemic changes in metabolism: long-term caloric restriction prevents them in old fibroblasts in a reversible manner, whereas a high-fat diet potentiates them in young fibroblasts. Finally, inhibition of the master regulator of adipogenesis, PPARgamma, attenuates fibroblast aging in vivo, providing potential anti-aging therapeutic alternatives to caloric restriction.
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| Overall design |
2-months-old female mice were fed for 6 months with a high fat diet or control diet. All mice were sacrificed on the same day. N=4
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| Contributor(s) |
Salzer M, Aznar-Benitah S |
| Citation(s) |
30415840 |
| Submission date |
Feb 22, 2018 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Antoni Berenguer-LLergo |
| E-mail(s) |
aberenguerl@tauli.cat
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| Organization name |
Parc Taulí Research and Innovation Institute (I3PT)
|
| Department |
A8G2 Group - Rheumatology Service
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| Street address |
Parc del Taulí, 1
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| City |
Sabadell |
| State/province |
Barcelona |
| ZIP/Postal code |
08208 |
| Country |
Spain |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (8)
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| GSM3019679 |
dermal fibroblasts from Normal Diet (ND) specimen 1 |
| GSM3019680 |
dermal fibroblasts from Normal Diet (ND) specimen 2 |
| GSM3019681 |
dermal fibroblasts from Normal Diet (ND) specimen 3 |
| GSM3019682 |
dermal fibroblasts from Normal Diet (ND) specimen 4 |
| GSM3019683 |
dermal fibroblasts from High Fat Diet (HFD) specimen 1 |
| GSM3019684 |
dermal fibroblasts from High Fat Diet (HFD) specimen 2 |
| GSM3019685 |
dermal fibroblasts from High Fat Diet (HFD) specimen 3 |
| GSM3019686 |
dermal fibroblasts from High Fat Diet (HFD) specimen 4 |
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| This SubSeries is part of SuperSeries: |
| GSE110983 |
The transcriptome of murine dermal fibroblasts |
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| Relations |
| BioProject |
PRJNA435492 |
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