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| Status |
Public on Nov 14, 2018 |
| Title |
The effect of caloric restriction on the transcriptome of dermal fibroblasts during aging |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
During aging, stromal functions are thought to be impaired, yet little is known whether this stems from molecular and cellular changes of fibroblasts, a major component of stroma. Using population- and single-cell whole transcriptomics, and long-term lineage tracing, we studied alterations in murine dermal fibroblasts during physiological aging under different dietary regimes known to affect longevity. We show that the identity of aged fibroblasts becomes undefined, with the distinct fibroblast states present in young skin no longer clearly demarcated. In addition, old fibroblasts not only reduce the expression of genes involved in the formation of the extracellular matrix, but intriguingly, also gain adipogenic traits, paradoxically becoming similar to neonatal pro-adipogenic fibroblasts. These age-related alterations are sensitive to systemic changes in metabolism: long-term caloric restriction prevents them in old fibroblasts in a reversible manner, whereas a high-fat diet potentiates them in young fibroblasts. Finally, inhibition of the master regulator of adipogenesis, PPARgamma, attenuates fibroblast aging in vivo, providing potential anti-aging therapeutic alternatives to caloric restriction.
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| Overall design |
9-months-old female mice were either fed a normal diet ad libitum for 9 months (Old ND group), a normal diet for 2 months followed by 30% caloric restriction for 7 months of (Old CR group), or a 30% caloric restriction for 7 months followed by a normal diet for 2 months (Old CR-ND group). Young mice (4-weeks-old) were fed ND ad libitum for 4 weeks (Young ND group). For sample collection three mice from different groups were sacrificed per day. n=5 (biological replicates)
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| Contributor(s) |
Salzer M, Aznar-Benitah S |
| Citation(s) |
30415840 |
| Submission date |
Feb 22, 2018 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Antoni Berenguer-LLergo |
| E-mail(s) |
aberenguerl@tauli.cat
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| Organization name |
Parc Taulí Research and Innovation Institute (I3PT)
|
| Department |
A8G2 Group - Rheumatology Service
|
| Street address |
Parc del Taulí, 1
|
| City |
Sabadell |
| State/province |
Barcelona |
| ZIP/Postal code |
08208 |
| Country |
Spain |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (20)
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| GSM3019687 |
dermal fibroblasts from ND/CR - Old specimen [1985 2016] |
| GSM3019688 |
dermal fibroblasts from ND - Old specimen [1986 2016] |
| GSM3019689 |
dermal fibroblasts from ND - Young specimen [1987 2016] |
| GSM3019690 |
dermal fibroblasts from CR/ND - Old specimen [1988 2016] |
| GSM3019691 |
dermal fibroblasts from ND/CR - Old specimen [1989 2016] |
| GSM3019692 |
dermal fibroblasts from ND - Young specimen [1990 2016] |
| GSM3019693 |
dermal fibroblasts from ND/CR - Old specimen [1991 2016] |
| GSM3019694 |
dermal fibroblasts from ND - Old specimen [1992 2016] |
| GSM3019695 |
dermal fibroblasts from ND - Young specimen [1993 2016] |
| GSM3019696 |
dermal fibroblasts from CR/ND - Old specimen [1994 2016] |
| GSM3019697 |
dermal fibroblasts from ND - Old specimen [1995 2016] |
| GSM3019698 |
dermal fibroblasts from ND/CR - Old specimen [1996 2016] |
| GSM3019699 |
dermal fibroblasts from CR/ND - Old specimen [1999 2016] |
| GSM3019700 |
dermal fibroblasts from ND - Young specimen [2075 2016] |
| GSM3019701 |
dermal fibroblasts from ND/CR - Old specimen [2076 2016] |
| GSM3019702 |
dermal fibroblasts from ND - Old specimen [2077 2016] |
| GSM3019703 |
dermal fibroblasts from CR/ND - Old specimen [2078 2016] |
| GSM3019704 |
dermal fibroblasts from ND - Old specimen [2079 2016] |
| GSM3019705 |
dermal fibroblasts from CR/ND - Old specimen [2080 2016] |
| GSM3019706 |
dermal fibroblasts from ND - Young specimen [2082 2016] |
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| This SubSeries is part of SuperSeries: |
| GSE110983 |
The transcriptome of murine dermal fibroblasts |
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| Relations |
| BioProject |
PRJNA435493 |
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