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Series GSE11107 Query DataSets for GSE11107
Status Public on Apr 09, 2008
Title Effect of starvation on the transcriptomes of the brain and liver in adult female zebrafish (Danio rerio)
Organism Danio rerio
Experiment type Expression profiling by array
Summary We used microarray analyses in adult female zebrafish (Danio rerio) to identify metabolic pathways regulated by starvation in two key organs that 1) serve biosynthetic and energy mobilizing functions (liver) and 2) consume energy and direct behavioral responses (brain). Starvation affected the expression of 574 transcripts in the liver, indicating an overall decrease in metabolic activity, reduced lipid metabolism, protein biosynthesis and proteolysis, and cellular respiration, and increased gluconeogenesis. Starvation also regulated expression of many components of the Unfolded Protein Response, the first such report in a species other than yeast (Saccharomyces cerevisiae) and mice (Mus musculus). The response of the zebrafish hepatic transcriptome to starvation was strikingly similar to that of rainbow trout (Oncorhynchus mykiss), but very different from common carp (Cyprinus carpio) and mouse. The transcriptome of zebrafish whole brain was much less affected than the liver, with only two differentially expressed genes, both down-regulated. Down-regulation of one of these genes, matrix metalloproteinase 9 (mmp9), suggests increased inhibition of apoptosis (neuroprotection) and decreased restructuring of the extracellular matrix in the brain of starved zebrafish. The low level of response in the transcriptome of whole zebrafish brain agrees with observations that the brain is metabolically protected compared to the rest of the body.
Keywords: starvation study
Overall design Brain treatments: Starved (21 days) and Control (Fed). Four microarrays per treatment.
Liver treatments: Starved (21 days) and Control (Fed). Five microarrays per treatment.
Data for brain and liver were normalized and analyzed separately because variances of expression differed considerably between the tissues.
Contributor(s) Drew RE, Rodnick KJ, Settles M, Wacyk J, Churchill E, Powell MS, Hardy RW, Murdoch GK, Hill RA, Robison BD
Citation(s) 18728227
Submission date Apr 09, 2008
Last update date May 30, 2018
Contact name Robert Edward Drew
Phone 208-885-8478
Fax 208-885-7905
Organization name University of Idaho
Department Biological Sciences
Lab Robison
Street address Life Sciences South Rm 252
City Moscow
State/province ID
ZIP/Postal code 83844-3051
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (18)
GSM280425 Danio_Brain_Control_1 (X2BC1)
GSM280426 Danio_Brain_Control_2 (X2BC2)
GSM280427 Danio_Brain_Control_3 (X2BC3)
BioProject PRJNA106993

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Supplementary file Size Download File type/resource
GSE11107_RAW.tar 34.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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