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| Status |
Public on Sep 06, 2018 |
| Title |
Transcriptional analysis of PPARgamma activation in control and PPARgamma null macrophages [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The nuclear receptor Peroxisome Proliferator Activated Receptor gamma (PPARgamma) is known to regulate lipid metabolism in many tissues, including macrophages. Here we report that macrophage respiration is enhanced by rosiglitazone, an activating PPARgamma ligand, in a PPARgamma dependent manner. Moreover, PPARgamma is required for macrophage respiration even in the absence of exogenous ligand. Unexpectedly, the absence of PPARgamma dramatically affects the oxidation of glutamine. Both glutamine and PPARgamma have been implicated in alternative activation (AA) of macrophages and PPARgamma was required for IL4-dependent gene expression and stimulation of macrophage respiration. Remarkably, depletion of PPARgamma phenocopied the effects of glutamine depletion on transcription. Thus, PPARgamma supports alternative activation by facilitating glutamine metabolism. Yet PPARgamma expression is itself markedly increased by IL4. Thus, PPARgamma functions at the center of a feed forward loop that is central to AA of macrophages.
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| Overall design |
RNA profiling of thioglycollate elicited macrophages harvested from four control and four PPARgammaKO mice. Untreated macrophages or macrophages treated for 24 hours with either DMSO, IL4, or Rosiglitazone
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| Contributor(s) |
Nelson VL, Nguyen HC, Briggs ER, Ho WY, Dispirito JR, Maranis JM, Hill DA, Lazar MA |
| Citation(s) |
30006480 |
| Submission date |
Feb 26, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Hoang Nguyen |
| E-mail(s) |
hoang.buinguyen@pennmedicine.upenn.edu
|
| Phone |
(215) 898-0198
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| Organization name |
University of Pennsylvania Perelman School of Medicine
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| Street address |
3400 Civic Center Boulevard
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104-5160 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (31)
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| This SubSeries is part of SuperSeries: |
| GSE111105 |
Transcriptional analysis of PPARgamma activation in control and PPARgamma null macrophages |
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| Relations |
| BioProject |
PRJNA436056 |
| SRA |
SRP133562 |
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