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Series GSE111104 Query DataSets for GSE111104
Status Public on Sep 06, 2018
Title Transcriptional analysis of PPARgamma activation in control and PPARgamma null macrophages [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The nuclear receptor Peroxisome Proliferator Activated Receptor gamma (PPARgamma) is known to regulate lipid metabolism in many tissues, including macrophages. Here we report that macrophage respiration is enhanced by rosiglitazone, an activating PPARgamma ligand, in a PPARgamma dependent manner. Moreover, PPARgamma is required for macrophage respiration even in the absence of exogenous ligand. Unexpectedly, the absence of PPARgamma dramatically affects the oxidation of glutamine. Both glutamine and PPARgamma have been implicated in alternative activation (AA) of macrophages and PPARgamma was required for IL4-dependent gene expression and stimulation of macrophage respiration. Remarkably, depletion of PPARgamma phenocopied the effects of glutamine depletion on transcription. Thus, PPARgamma supports alternative activation by facilitating glutamine metabolism. Yet PPARgamma expression is itself markedly increased by IL4. Thus, PPARgamma functions at the center of a feed forward loop that is central to AA of macrophages.
 
Overall design Immuno-precipitation followed by high throughput sequencing of thioglycollate elicited macrophages harvested from four control and four PPARgammaKO mice.
 
Contributor(s) Nelson VL, Nguyen HC, Briggs ER, Ho WY, Dispirito JR, Maranis JM, Hill DA, Lazar MA
Citation(s) 30006480
Submission date Feb 26, 2018
Last update date Mar 19, 2019
Contact name Hoang Nguyen
E-mail(s) hoang.buinguyen@pennmedicine.upenn.edu
Phone (215) 898-0198
Organization name University of Pennsylvania Perelman School of Medicine
Street address 3400 Civic Center Boulevard
City Philadelphia
State/province PA
ZIP/Postal code 19104-5160
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM3022231 KO_pool [ChIP-seq]
GSM3022232 WT_pool [ChIP-seq]
This SubSeries is part of SuperSeries:
GSE111105 Transcriptional analysis of PPARgamma activation in control and PPARgamma null macrophages
Relations
BioProject PRJNA436057
SRA SRP133564

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Supplementary file Size Download File type/resource
GSE111104_RAW.tar 140.7 Mb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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