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| Status |
Public on Jun 01, 2008 |
| Title |
Mouse liver gene expression after 4.5 days of repeated combined acoustic and restraint stress vs. control |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Stress is a powerful modulator of neuroendocrine, behavioral and immunological functions. After 4.5 days of repeated combined acoustic and restraint stress as a murine model of chronic psychological stress severe metabolic dysregulations became detectable in female BALB/c mice. Stress-induced alterations of metabolic processes that were found in a hepatic mRNA expression profiling were verified by in vivo analyses. Repeatedly stressed mice developed a hypermetabolic syndrome with severe loss of lean body mass, hyperglycemia, dyslipidemia, increased amino acid turn-over, and acidosis. This was associated with hypercortisolism, hyperleptinemia, insulin resistance, and hypothyroidism. In contrast, after a single acute stress exposure changes in expression of metabolic genes were much less pronounced and predominantly confined to gluconeogenesis, probably indicating that metabolic disturbances might be initiated already early but will only manifest in repeatedly stressed mice .Thus, in our murine model, repeated stress caused severe metabolic dysregulations leading to a drastic reduction of the individual's energy reserves. Under such circumstances stress may further reduce the ability to cope with new stressors such as infection or cancer.
Keywords: stress response
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| Overall design |
Two biological experiments of repeated combined acoustic and restraint stress were performed, which consist each of a repeatedly stressed group and an untreated control group. Liver RNA expression profiles were analyzed in technical duplicates using pools of 8 or 9 individual RNAs of each group.
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| Contributor(s) |
Depke M, Geffers R, Völker U, Schütt C, Kiank C |
| Citation(s) |
18325986 |
| Submission date |
Apr 10, 2008 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Maren Depke |
| Organization name |
Ernst-Moritz-Arndt-University Greifswald, Interfaculty Institute of Genetics and Functional Genomics
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| Department |
Department of Functional Genomics
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| Street address |
F.-L.-Jahn-Str. 15a
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| City |
Greifswald |
| ZIP/Postal code |
17489 |
| Country |
Germany |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (8)
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| GSM280631 |
Liver_repeated stress_bio rep 1_tech rep 1 |
| GSM280632 |
Liver_repeated stress_bio rep 1_tech rep 2 |
| GSM280633 |
Liver_repeated stress_bio rep 2_tech rep 1 |
| GSM280634 |
Liver_repeated stress_bio rep 2_tech rep 2 |
| GSM280635 |
Liver_control for repeated stress_bio rep 1_tech rep 1 |
| GSM280636 |
Liver_control for repeated stress_bio rep 1_tech rep 2 |
| GSM280637 |
Liver_control for repeated stress_bio rep 2_tech rep 1 |
| GSM280638 |
Liver_control for repeated stress_bio rep 2_tech rep 2 |
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| This SubSeries is part of SuperSeries: |
| GSE11126 |
Expression data from mouse liver after combined acoustic and restraint stress compared to non-stressed control mice |
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| Relations |
| BioProject |
PRJNA109175 |
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