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| Status |
Public on Mar 13, 2018 |
| Title |
Blood and skeletal muscle gene signatures of exercise training in men (PBMC data set) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Accounting for transcriptional features of endurance adaptations to training is important and could help elucidate the high variability in oxygen uptake (VO2) response. We aimed to identify whole-transcriptome signatures of an endurance training protocol in whole-blood (leukocytes), PBMCs and skeletal muscle tissue of the same group of individuals in a controlled environment.
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| Overall design |
Two groups of healthy untrained policemen recruits (G1, n=20, 26±4 yrs; G2, n= 21, 27±3 yrs) were selected. Blood (PAXgene and EDTA tubes) and muscle tissueforgene expression assays and peak VO2peak measures by cardiopulmonary exercise test were taken before and after 18 weeks of running (3 times/wk; 60min). RNA was extracted from whole-blood (leukocytes), PBMCs and muscle and was used for whole-genome expression analysis using AffymetrixGene Chip Human Transcriptome Array 2.0. Differentially expressed transcriptswere identified in muscle (547; p<0.01), PBMCs (876; p<0.000001) and leukocytes (175; p<0.0005). We identified significant correlation among muscle and blood tissues, as well as between repeated measures of transcriptomes from the same individual. Using sPLS analysis we derived a leukocyte gene-based classifier that was predictive of VO2peak status (pre and post-training) in G1 and was later validated in G2. Enrichment analysis of the genes present in the final classifier was concordant with previous, per-tissue, analysis and a clear enrichment of mitochondrial oxidative process was evident.
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| Contributor(s) |
Silva MS, Pereira AC |
| Citation missing |
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| Submission date |
Mar 08, 2018 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Michelle Sabrina Moreira Silva |
| E-mail(s) |
missabrina@gmail.com
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| Phone |
551126615511
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| Organization name |
Heart Institute - InCor
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| Street address |
Av. Dr. Enéas de Carvalho Aguiar
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| City |
São Paulo |
| State/province |
SP |
| ZIP/Postal code |
05403-900 |
| Country |
Brazil |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (32)
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| This SubSeries is part of SuperSeries: |
| GSE111555 |
Blood and skeletal muscle gene signatures of exercise training in men |
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| Relations |
| BioProject |
PRJNA437853 |
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